The invention discloses a method for accurately simulating the response of a grassland soil seed bank to soil temperature increase, relates to a method for simulating the response of a soil seed bank to soil temperature increase and aims at solving the problem that a traditional heating tube method is non-uniform in soil temperature change, is easily unstable and cannot accurately quantify temperature increase amplitude. The method comprises the steps that 1, the soil seed bank is sampled; 2, a germination utensil is punched; 3, germination utensil holes are protected; 4, a carbon fiber heating cable is laid; 5, a culture medium is charged; 6, a temperature probe is buried; 7, the heating cable is connected with the temperature probe; 8, the temperature increase amplitude is set; 9, the soil seed bank sample is spread; 10, conventional watering management is performed. The method adopts the simple tools, is convenient to operate and remarkable in effect, can accurately increase the temperature increase amplitude, performs automatic power off after the temperature is reached and is used for simulating the response of the soil seed bank to the soil temperature increase.