Rapid germination method of soil seed bank

A soil seed bank, a fast technology, applied in application, cultivation, agriculture, etc., can solve the problems of increasing the number of experiments, difficult germination conditions, and difficulty in recording experiments, achieving low production costs, easy handling and storage, and easy repetition. The effect of the experiment

Inactive Publication Date: 2010-04-21
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the germination of different seeds has different requirements for humidity, temperature, light, and salinity, and it is difficult to meet the optimal germination conditions for all species in the experiment.
In order to reduce this experimental error, some auxiliary measures can be added before the germination of the soil sample, such as: concentrate the soil sample, disinfect the soil sample, break the dormancy, mix the soil sample with an appropriate amount of water-retaining agent, and use wet treatment. Methods, covering soil samples with light-transmitting isolation nets, prolonging the duration of the experiment and increasing the number of repetitions, these treatments will result in a substantial increase in the number of experiments, and require that the germination device needs to be easy to move. These are the trays used to carry out germination experiments in the past. Research institutes are difficult to solve, for example: a sample of germination needs 6-7 times of treatment, each treatment needs 6-9 times of repetition, different treatments need to be placed in the laboratory for a while, and need to be in the field for a while, and then Each treatment needs 7-9 water, so each sampling may need to take 60-100 samples, and the treatment and repetition of each sample requires 210-300 simultaneous repetitions. Each experiment needs nearly 10,000 trays. Handling takes hours and often causes difficulties in recording experiments and repeated handling errors

Method used

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  • Rapid germination method of soil seed bank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 (single germination box)

[0020] Soil sample collection and processing: A specially made wooden frame (with an inner diameter of 40 cm × 40 cm) was used to place the sample on the sample square to prevent the surrounding soil from slipping. Use a metal spatula to take 5 cm of undisturbed soil, and the collected soil sample Take it back to the laboratory, mix the same soil sample in the same soil layer, sieve it with a soil sieve with an aperture of 2 mm, and fully mix the sieved soil sample;

[0021] The making of the germination box: use a plank with a thickness of 1 cm to make a square germination box (1) of 10 cm × 10 cm × 5 cm, seal the bottom with a plank with a thickness of 1 cm, and open 3 diameters on the bottom plate (2). The circular hole (3) is 2-5 mm, the purpose is to allow the supplementary water to infiltrate naturally, avoiding the amount of water collected in the soil sample and affecting its seed germination;

[0022] The laying of soil ...

Embodiment 2

[0025] Embodiment 2 (combined germination wooden box)

[0026] Soil sample collection and processing: A specially made wooden frame (with an inner diameter of 40 cm × 40 cm) was used to place the sample on the sample square to prevent the surrounding soil from slipping. Use a metal spatula to take 5 cm of undisturbed soil, and the collected soil sample Take it back to the laboratory, mix the same soil sample in the same soil layer, sieve it with a soil sieve with an aperture of 2 mm, and fully mix the sieved soil sample;

[0027] Combined germination box production: a single germination box is placed on the same wooden board for production, the specification is 100 cm x 100 cm x 5 cm, and the combined germination device (6) containing 100 germination boxes (1), the specific number can be Determined according to the experimental situation;

[0028] The bottom is sealed with a 1 cm thick wooden board, and 3 round holes (3) with a diameter of 2-5 mm are opened on the bottom boar...

Embodiment 3

[0032] Embodiment 3 (germination experiment in the field)

[0033]The field experiment site is located in the 33rd regiment in the lower reaches of the Tarim River, located on the northeastern edge of the Tarim Basin, with geographic coordinates of 87°13′E, 40°47′N;

[0034] Soil sample collection and processing: A specially made wooden frame (with an inner diameter of 40 cm × 40 cm) was used to place the sample on the sample square to prevent the surrounding soil from slipping. Use a metal spatula to take 5 cm of undisturbed soil, and the collected soil sample Take it back to the laboratory, mix the same soil sample in the same soil layer, sieve it with a soil sieve with an aperture of 2 mm, and fully mix the sieved soil sample;

[0035] Select an experimental site as a soil seed bank germination bed, the germination bed is composed of n germination boxes arranged side by side, and the distance between the germination beds is 2 meters;

[0036] When laying soil samples in th...

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Abstract

The invention relates to a rapid germination method of soil seed bank and is achieved by the following steps of making a germination box, laying a soil sample, compensating soil moisture, confirming a germinated seedling and the like. The method solves the technical problem of carrying out the germination of the soil seed bank in large scale under controlled conditions, improves the accuracy and the scientificity of the germination experiment of the soil seed bank, has the advantages of high experimental efficiency and precision, short germination time, easy seedling identification and the like, and can be popularized in and applied to different areas.

Description

technical field [0001] The invention belongs to the technical field of restoration of damaged ecosystems, and in particular relates to a soil seed bank germination technology in arid areas. Background technique [0002] The soil seed bank refers to the sum of all viable seeds remaining in the litter on the soil surface and in the soil. It is a research hotspot in the interdisciplinary fields of ecology, physical geography, botany, genetics, weed science and agronomy. The research on seed banks in foreign countries has a history of more than 150 years since Darwin. Seed banks can participate in the renewal of aboveground vegetation, which is the basis for population settlement, survival, reproduction and dispersal, and plays an important role in the maintenance of aboveground vegetation community structure and species diversity. Prediction, this has been widely recognized. In view of the important role of soil seed bank in ecological restoration, soil seed bank has attracted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G9/10
Inventor 徐海量叶茂张鹏王炜张占江张青青
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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