Compound plant extract feed additive and preparation method thereof
A feed additive and plant extract technology, which is applied in the field of compound plant extract feed additive and its preparation, can solve problems such as the need for time, and achieve the effect of good in vitro antibacterial activity
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Embodiment 1
[0033] (1) Citrus essential oil - distillation method
[0034] Dried orange peel - crushed - steam distillation - orange peel essential oil.
[0035] After the citrus is dried, it is crushed to 40 mesh orange peel powder. The citrus powder and water are added to the distiller according to the solid-to-liquid ratio of 1:5. When NaCL2.0% is added and the distillation time is 60 minutes, the purified orange peel essential oil is obtained at the distillation place.
[0036] (2) Extraction of citrus flavonoids
[0037] Most flavonoids have phenolic hydroxyl groups, so they can be leached with alkaline water or alkaline dilute alcohol, and the flavonoids will be separated out after the leachate is acidified.
[0038] Extraction equipment: Reagents and equipment: rutin, anhydrous methanol, hydrochloric acid, sodium nitrite, aluminum nitrate, sodium hydroxide, etc. Electrothermal constant temperature blast drying oven, spectrophotometer, high-speed pulverizer, electronic balance, ac...
Embodiment 2
[0049] Embodiment 2 Composite plant extract feed additive of the present invention antibacterial effect in vitro
[0050] Aeromonas hydrophila (Aeromonas hydrophila) belongs to Vibriaceae and Aeromonas genus. It is a mesophilic and dynamic Aeromonas group. Extensive pathogenicity, pathogenic to aquatic animals, amphibians and mammals. In aquatic products, it is the most common pathogenic bacteria in fish. The infection of freshwater fish is called bacterial sepsis, with a morbidity rate of over 90% and a mortality rate of over 65%. It was widely popular in more than 20 provinces, municipalities, and autonomous regions including Shanghai, Jiangsu, Zhejiang, Anhui, Guangdong, Guangxi, and Fujian. A variety of freshwater farmed fish such as California bass, shrimp, and soft-shelled turtle. The application of plant extracts in aquatic products as immune enhancers and therapeutic drugs is gradually increasing.
[0051] The present invention selects 5 kinds of extracts of 4 kinds...
Embodiment 3
[0082] Example 3 Extraction process optimization of natural plant active ingredients such as citrus peel, pine needles, pollen and garlic
[0083] 1. Extraction method and optimization of citrus essential oil
[0084] Extraction methods of citrus essential oil: pressing method, distillation method, solvent extraction method, microwave or ultrasonic assisted extraction and supercritical CO 2 Extraction method, etc., pressing method is suitable for fresh raw materials, and organic solvent extraction not only extracts some pigments, flavonoids and other substances at the same time, but also emulsion may appear later. Supercritical CO 2 The extraction method has high operating costs and expensive equipment. The present invention considers comprehensively and uses distillation to optimize the citrus essential oil.
[0085] Material: Ripe and fresh citrus, washed and peeled
[0086] Equipment: volatile oil extraction device, conventional steam distillation device.
[0087] Test...
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