Method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears

A technology of thin-layer cells and acid-fast bacilli, which is applied in the preparation of test samples, measuring devices, and material analysis through optical means, can solve problems such as complicated operations, slow results, and long separation and culture times, and achieve relief. Effects of patient burden, spread control, and positive rate of acid-fast bacilli

Inactive Publication Date: 2014-05-28
吉安协和医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microscopic examination of acid-fast staining of smears is a widely used technique in the bacteriological examination of tuberculosis worldwide. This method is simple in equipment and easy to operate, but the positive rate of detection is low (about 5%-10%), and it has shown a downward trend in recent years
Separation culture is more sensitive and specific than smear acid-fast staining microscopic examination, but requires high equipment, complicated operation, long culture time, and it takes 2 months to report the results, which cannot meet the needs of clinical diagnosis
In order to break through the limitations of low positive rate of acid-fast staining microscopic examination of smears, long separation and culture time, and slow results, domestic and foreign have been discussing simple and practical inspection methods with high positive rate, but no successful reports have been found.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0020] (1) Collect specimens. Collect 2-5ml of sputum from the subject;

[0021] (2) Prepare the treatment solution. Liquid-based thin-layer cell smear test acid-fast bacilli sputum pretreatment solution prepared by ourselves;

[0022] (3) Centrifugal. The above-mentioned sputum is added to the self-made sputum pre-treatment solution, shaken for 20-30 minutes, centrifuged at 2000 rpm, and taken out after centrifugation for 2-10 minutes;

[0023] (4) Production. Use a straw to absorb 1-2 ml of sputum from the bottom of the sputum pretreatment solution test tube after shaking and centrifugation, and put it into the film-making chamber. Put the film-making chamber into the film-making machine, take it out and dry after 6-10 minutes after the film-making mechanism is completed;

[0024] (5) For acid-fast staining, after the smear is dry, heat and fix it, add phenolic fuchsin staining solution dropwise, keep staining for 5-10 minutes, wash with light buffer under running water,...

specific Embodiment 2

[0027] (1) Collect specimens. Collect 2-5ml of cerebrospinal fluid from the subject;

[0028] (2) Prepare the treatment solution. Liquid-based thin-layer cell smear test acid-fast bacilli sputum pretreatment solution prepared by ourselves;

[0029] (3) Centrifugal. The above cerebrospinal fluid sputum is added to the self-made sputum pretreatment solution, shaken for 5-10 minutes, centrifuged at 2000 rpm, and taken out after centrifugation for 2-10 minutes;

[0030] (4) Production. Use a straw to absorb 1-2 ml of cerebrospinal fluid at the bottom of the sputum pretreatment solution test tube after vibration and centrifugation, and put it into the film-making chamber, which is put into the film-making machine. After 6-10 minutes, take out the film-making machine and dry it. ;

[0031] (5) For acid-fast staining, after the smear is dry, heat and fix it, add phenolic fuchsin staining solution dropwise, keep staining for 5-10 minutes, wash with light buffer under running wate...

specific Embodiment 3

[0034] (1) Collect specimens. Take 2-5ml of bronchial washing fluid from the subject;

[0035] (2) Prepare the treatment solution. Liquid-based thin-layer cell smear test acid-fast bacilli sputum pretreatment solution prepared by ourselves;

[0036] (3) Centrifugal. The above-mentioned bronchial washing liquid sputum is added to the self-made sputum pretreatment liquid, shaken for 10-20 minutes, centrifuged at 2000 rpm, and taken out after centrifugation for 2-10 minutes;

[0037] (4) Production. Use a straw to absorb 1-2 ml of the bronchial flushing solution at the bottom of the sputum pretreatment solution test tube after shaking and centrifugation, and put it into the film-making chamber, which is put into the film-making machine. After 6-10 minutes, the film-making mechanism is completed. Take out and dry;

[0038] (5) For acid-fast staining, after the smear is dry, heat and fix it, add phenolic fuchsin staining solution dropwise, keep staining for 5-10 minutes, wash ...

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Abstract

The invention discloses a method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears. The method comprises the following steps: collecting a specimen, and collecting 2-5ml of sputum of a subject; preparing a treating liquid, and preparing a sputum pretreating liquid for inspecting acid-fast bacilli by the liquid-based thin-layer cell smears; centrifuging, adding the sputum into the prepared sputum pretreating liquid, and vibrating for multiple seconds; preparing a section, absorbing multiple milliliters of the sputum at the bottom of a sputum pretreating liquid test tube by a sucker, putting the sputum into a section preparation bin, putting the section preparation bin into a section preparation machine, and taking out the section preparation bin to dry when the section preparation machine completes the preparation; and carrying out acid-fast staining and microscopic inspection. Compared with the prior art, the method provided by the invention has the advantages that the positive incidence for inspecting acid-fast bacilli by sputum thick smears is improved by tens of times; the tuberculosis infection source can be found out as soon as possible; the tuberculosis can be treated early if being found out early; the burden of patients is relieved; the tuberculosis expansion can be controlled.

Description

technical field [0001] The invention relates to a method for checking acid-fast bacilli, in particular to a method for checking acid-fast bacilli with a liquid-based thin-layer cell smear. Background technique [0002] Tuberculosis is a chronic infectious disease that seriously endangers human health. In tuberculosis control work, tuberculosis bacteriological examination is of great significance for discovering the source of infection, confirming diagnosis and formulating treatment plan, and is the gold standard for diagnosing tuberculosis. Tuberculosis bacteriological examination currently includes smear acid-fast staining microscopic examination and isolation culture. Microscopic examination of acid-fast staining of smears is a widely used technique in the bacteriological examination of tuberculosis worldwide. This method is simple in equipment and easy to operate, but the positive rate of detection is low (about 5%-10%), and it has shown a downward trend in recent years ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N21/84
Inventor 胡望福周小玫詹国苍罗智勇刘高焦陈学峰段水莲刘子东肖人鸿彭江琴
Owner 吉安协和医院
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