Immune test paper for detecting group A rotaviruses, and its making method

A technology of rotavirus and porcine rotavirus, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation process, time-consuming, and troublesome detection of group A rotavirus

Inactive Publication Date: 2014-07-02
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The diagnosis of PoRV in my country mainly relies on routine laboratory diagnosis. The operation process is complex, time-consuming, and requires expensive instruments, which makes the detection of group A rotavirus more troublesome.

Method used

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  • Immune test paper for detecting group A rotaviruses, and its making method
  • Immune test paper for detecting group A rotaviruses, and its making method

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Embodiment Construction

[0022] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. A preferred embodiment of the invention is shown in the drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of the present invention will be thorough and complete.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0024] Please...

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Abstract

An immune test paper for detecting group A rotaviruses comprises a sample pad, a bonding pad, a cellulose nitrate film, a water absorbing pad and a back lining, the sample pad, the bonding pad, the cellulose nitrate film and the water absorbing pad are boned on the back lining, two ends of the bonding pad have a lapping joint with the sample pad and the cellulose nitrate film respectively, one end of the cellulose nitrate film far from the binding pad has a lapping joint with the water absorbing pad, the cellulose nitrate film is provided with a detection line and a quality control line which are spaced, the bonding pad is provided with a group A porcine rotavirus monoclonal antibody A-fluorescent marker, the detection line is formed by a group A monoclonal porcine rotavirus antibody B, and the quality control line is formed by goat anti-mouse IgG. The invention also provides a making method of the immune test paper for detecting group A rotaviruses.

Description

technical field [0001] The invention relates to a group A rotavirus detection immunity test paper and a preparation method thereof. Background technique [0002] At present, porcine rotavirus (Porcine rotavirus, PoRV) belongs to the genus Rotavirus of the family Reoviridae, and rotavirus infection is an acute intestinal infectious disease that causes digestive tract dysfunction in piglets. It is characterized by disturbance of acid-base balance and brings serious harm to the pig industry. There are many serotypes of PoRV. The rotavirus infection in pigs in my country is dominated by group A. In group A PoRV, VP6 protein is the group antigen, which is highly conserved among the strains. The diagnosis of PoRV in my country mainly relies on routine laboratory diagnosis, the operation process is complex, time-consuming, and requires expensive instruments, which makes the detection of group A rotavirus more troublesome. Contents of the invention [0003] Based on this, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/56983G01N33/558G01N2333/14
Inventor 万晓春任广慧金言
Owner SHENZHEN INST OF ADVANCED TECH
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