A kind of breeding method of radish maintainer line with ogura male sterile cytoplasm
A male sterile line, male sterile technology, applied in the direction of botany equipment and methods, biochemical equipment and methods, microbial determination/inspection, etc.
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[0035] The present invention will be described in detail below in conjunction with the accompanying drawings and implementation examples.
[0036] First select the male sterile material and the proposed maintainer line material, the male sterile material selected in the present invention is RBY imported from abroad, and RBY is the type of Japanese green radish; the proposed maintainer line material is Shandong local variety Zaozhuang Dahongpao, Zaozhuang Dahong The gown is red-skinned and white-hearted radish. After selecting the material, carry out the PCR molecular marker and fragment sequencing of the cytoplasmic type. The sterile line is required to have OguraH-type cytoplasm, and the maintainer line has OguraA-type cytoplasm.
[0037] Ogura-specific primers: ORF-F5'-TTCAAATCCTGTCCCCGCACC-3' and ORF-R5'-GCCTTACACCATTGGGATACTTC-3' were used to carry out PCR molecular markers on the sterile material RBY and the proposed maintainer line material Zaozhuang Dahongpao. In order...
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