Use of 2α-hydroxy protopanaxadiol in the preparation of tumor multidrug resistance reversal agent

A technology of panaxadiol and drug resistance reversal, which is applied in the field of medicine to achieve the effects of reversing drug resistance characteristics, enhancing therapeutic effects, and inhibiting tumors

Inactive Publication Date: 2017-10-10
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report about the regulation of mogroside 2α-hydroxyprotopanaxadiol on the drug resistance of tumor cells

Method used

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  • Use of 2α-hydroxy protopanaxadiol in the preparation of tumor multidrug resistance reversal agent
  • Use of 2α-hydroxy protopanaxadiol in the preparation of tumor multidrug resistance reversal agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Implementation 1: CCK-8 kit detects drug-resistant cell line resistance to 2α-hydroxyprotopanaxadiol

[0047] Experimental Materials:

[0048] 2α-Hydroxyprotopanaxadiol is extracted from the plant Luo Han Guo with a purity of not less than 95%. Human oral cancer cell line KB and its drug-resistant cell line KB / VCR were provided by the Chinese Academy of Sciences, human breast cancer cell line MCF-7 and its drug-resistant cell line MCF-7 / ADR, human colon cancer HCT-8 and its drug-resistant cell line Drug cell line HCT-8 / VCR were purchased from Nanjing Kaiji Biological Company. Verapamil (VPL), vincristine (VCR) and doxorubicin (ADR) were purchased from Roche Chemical Company with a purity greater than 99%. CCK-8 kit was purchased from Tongren Company.

[0049] experimental method:

[0050] Cell recovery

[0051] 1) Take out the cryotube from the liquid nitrogen tank, put it directly into warm water at 37°C, and shake it from time to time to melt it as soon as possib...

Embodiment 2

[0075] Example 2: CCK-8 method detects the reversal effect of 2α-hydroxyprotopanaxadiol on the multidrug resistance activity of tumor cells

[0076] Experimental material: with embodiment 1.

[0077] experimental method:

[0078] 2α-Hydroxyprotopanaxadiol sensitization experiment: KB / VCR, MCF-7 / ADR and HCT-8 / VCR cells were inoculated into 96-well plates at a density of 3500 / well, and after 24 hours, different concentrations of VCR and different concentrations The VCR and 2α-hydroxyprotopanaxadiol were prepared together in α-MEM containing 10% fetal bovine serum and added to each well. After culturing for 48 hours, the culture medium was discarded, and the CCK-8 kit was used to measure the activity of the drug-resistant cell line and its parental cells to VCR and VCR+2α-hydroxy protopanaxadiol in the same manner as in Example 1, and a curve was drawn. Three replicate wells were set up for each concentration, and the experiment was repeated three times.

[0079] Experimental ...

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Abstract

The invention belongs to the field of medicine, and relates to the application of 2α-hydroxyprotopanaxadiol in the preparation of antitumor drugs, especially the application of 2α-hydroxyprotopanaxadiol in the preparation of antitumor drug resistance reversal agents. 2α-Hydroxyprotopanaxadiol is a natural product that can reverse the multidrug resistance of tumor cells and can be used as a reversal agent for tumor multidrug resistance; 2α-Hydroxyprotopanaxadiol can also increase the resistance of tumor multidrug-resistant cells The role of tumor drug sensitivity can be used as a chemosensitizer. The invention also provides a medicine method for inhibiting the proliferation of tumor multidrug-resistant cells by using the pharmaceutical composition combined with antineoplastic drugs and 2α-hydroxy protopanaxadiol. The small molecule compound 2α-hydroxy protopanaxadiol in the present invention is developed as a new anti-tumor drug or its auxiliary components, and has obvious tumor-suppressing effect and is environmentally friendly, which will provide a new way and means for treating and curing tumors .

Description

technical field [0001] The invention belongs to the medical field of reversing tumor multidrug resistance and sensitizing antitumor drugs, and specifically relates to the new application of mogrogenin-2α-hydroxyl protopanaxadiol, that is, 2α-hydroxyl protopanaxadiol is used in the preparation of tumor multi-drug Application in drug reversal agent and antitumor drug sensitizer. Background technique [0002] Malignant tumors seriously endanger human life and health, and have become one of the main causes of death from diseases today. At present, the main treatment methods for malignant tumors include surgery, radiotherapy and chemotherapy. Chemotherapy is a better option for patients diagnosed with advanced tumors and those with metastases. Clinicians can optimize the maximum curative effect and minimum toxicity by adjusting the dosage of drugs, helping patients prolong survival time and improve the prognosis of life quality. However, the generation of drug resistance of tu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/575A61P35/00
Inventor 余龙朱恒锐胡立宏刘军华
Owner FUDAN UNIV
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