A Liver Cell Microsphere Circulation Bioartificial Liver Support System

Abstract

The invention relates to a bioartificial liver supporting system which comprises a blood circulation system, a plasmapheresis circulation system and a hepatocyte microsphere circulation system. An integrated blood oxygen, pH, blood pressure and temperature sensor is adopted for not only monitoring various physiological parameters in the system in real time, but also reducing the complication degree of the whole system, and facilitating data management; furthermore, a hepatocyte microsphere culture fluid storage device and a recycling method are adopted, so that not only can nutrient substances and the oxygen transmitting capacity be improved and can durable functions and activity of hepatocytes be maintained, but also full contact between the hepatocyte and plasma is facilitated, the bioartificial liver supporting system is capable of substituting the liver function of a patient with hepatic failure in a long time; furthermore, the hepatocyte microsphere culture fluid storage device is provided with a culture fluid extraction port and a culture fluid adding port, so that under the condition of not influencing normal operation of the system, cell samples can be extracted, the cell activity is detected and a fresh culture fluid containing hepatocyte microspheres is added.

Description

technical field [0001] The invention relates to the technical field of medical equipment, in particular to a liver cell microsphere circulation bioartificial liver support system. Background technique [0002] Bioartificial liver is the product of the combination of hepatocyte culture technology and blood purification technology. Its basic principle is to place the hepatocytes cultured and proliferated in vitro in a special bioreactor, and use the extracorporeal circulation device to transfer the blood of patients with liver failure or Plasma is introduced into the bioreactor, and undergoes material exchange and biological action with the liver cells through the semi-permeable membrane in the reactor. It uses liver cells to secrete endogenous active substances and transform exogenous toxins to play a role. Endogenous active substances include various proteins, metabolic enzymes and active factors. The bioartificial liver is the closest artificial liver support system to the...

AI Technical Summary

Problems solved by technology

This technology can theoretically achieve a certain liver function, but there are some shortcomings: 1) It cannot monitor the operating status of the artificial liver support system in real time, so it cannot detect and deal with problems in the system in time

In addition, the use of a certain type of sensor results in a wide variety of sensors, which not only is not conducive to data processing, but also makes the structure of the system complex.

2) The liver cells in the outer cavity of the bioreactor can only be maintained for a short time due to the lack of scaffold cell activity, and can only replace the liver function of patients with hepatic insufficiency for a short time

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