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Breeding method of crassostrea gigas with high melanin content in soft parts

A technology of growing oysters and melanin, applied in the field of breeding, can solve the problems of no report on the correlation between shell color and mantle color, great influence of subjective factors, and high cost of breeding, so as to improve the utilization value, improve the economic benefits of breeding, The effect of accelerating the breeding process

Active Publication Date: 2015-02-25
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some scholars have reported the breeding of long oysters with different mantle colors, but there is no report on the breeding of oysters with black shells on the left and right sides, and the breeding of long oysters with different mantle colors is based on the observation of mantle color with naked eyes. The internal correlation between shell color and mantle color has not been reported. This method needs to pry open the shell, which is greatly affected by subjective factors, laborious, laborious, high in breeding costs, and poor in accuracy.

Method used

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  • Breeding method of crassostrea gigas with high melanin content in soft parts
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  • Breeding method of crassostrea gigas with high melanin content in soft parts

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specific Embodiment approach

[0013] The specific embodiment: the specific embodiment of the present invention is described in detail below:

Embodiment

[0014] Embodiment: a kind of breeding method of long oysters containing a large amount of melanin in soft body selected by shell color, and the proof scheme of method effectiveness, it comprises the following steps:

[0015] 1.1 Breeding method:

[0016] (1) Observe and select oysters whose left and right shells are black, measure the surface area of ​​the oyster's left and right shells and the surface area of ​​the black shell part, and select oysters whose black shell area accounts for more than 90% of the total shell area.

[0017] (2) Use ND-2 NCS lightness meter to measure the lightness value of oyster shell color, and select oysters with lightness value Vfigure 1 ).

[0018] (3) The long oysters whose black surface area ratio and shell color brightness meet the requirements are reserved as seed shells for normal nursery operations.

[0019] 1.2 Proof of method validity:

[0020] (1) Extract the melanin in the shell from the selected oysters.

[0021] (2) Extract the ...

experiment example

[0023] Examples of experiments to prove the effectiveness of specific methods are as follows:

[0024] 1. Experimental materials:

[0025] Long oysters: The live long oysters used in this experiment were purchased from the Huanhai Road Farmer's Market in Yantai City, with an average weight of 100g and a total of 30 oysters.

[0026] 1.1 Instruments and equipment:

[0027] Circulating water vacuum pump, electronic density balance, electric heating constant temperature water bath, electric heating constant temperature water bath, electric heating constant temperature blast drying oven, a set of Soxhlet extractor, mortar, beaker, triangular flask, glass rod;

[0028] 1.2 Experimental reagents:

[0029] Including hydrochloric acid, ether, distilled water. Preparation of hydrochloric acid solution: The purchased hydrochloric acid has a standard concentration of 12mol / l, and the concentration of hydrochloric acid required in this experiment is 6mol / l. The hydrochloric ac...

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Abstract

The invention discloses a breeding method of crassostrea gigas (black oysters) with a high melanin content in the soft parts and a proof scheme for the effectiveness of the method. The breeding method includes the following steps that by observing the color of common oysters, the oysters of which the ratio of the area of black shells to the total shell area is more than 90 percent and the lightness value V< 0.20 are selected, melanin extraction of oyster shells, melanin extraction of soft parts of the oyster shells and relation analysis of the melanin content and the melanin content of the soft parts are carried out, the applicant finds a significant positive correlation phenomenon of the color of the shells of the crassostrea gigas and the deposit rate of melanin in the pallium in the experiment process, and the blacker the shells are, the higher the melanin content of the soft parts is. By means of the breeding method of the crassostrea gigas (black oysters) with the high melanin content in the soft parts, the oysters with the high melanin content in the soft parts can be selected and bred rapidly, the breeding efficiency of the oysters is improved, and as the health-care value of melanin is accepted by the market, the new oyster species obtained through the method can increase the additional value of breeding.

Description

[0001] Technical field: [0002] The invention relates to a breeding method, in particular to a breeding method of long oysters with high melanin content in the soft body ("black" oysters). Background technique: [0003] Melanin is a biological polymer widely distributed in animals and plants. With the continuous in-depth research on natural melanin, it has been found that melanin has a wide range of biological activities, including delaying aging, regulating immunity, protecting light damage, anti-oxidation, scavenging free radicals, protecting cardiovascular function, etc. Natural melanin with high content The food has been fully recognized by consumers and has a bright market prospect. [0004] At present, some scholars have reported the breeding of long oysters with different mantle colors, but there is no report on the breeding of oysters with black shells on the left and right sides, and the breeding of long oysters with different mantle colors is based on the observa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/54
Inventor 王晓通于文超赵南南张子豪王娇武长路
Owner LUDONG UNIVERSITY
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