Lignans isolated from Acorus calamus, its preparation and application
A technology of lignans and calamus, applied in ether separation/purification, ether preparation, organic chemistry, etc., can solve problems such as undisclosed extracts
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[0027] 1. The method of extracting and separating tatarinanS-T from the rhizome of Acorus calamus:
[0028] 10 kg of the traditional Chinese medicine Shichangpu was pulverized, extracted twice with 95% ethanol under reflux, combined the two extracts, concentrated under reduced pressure, and dried in vacuum to obtain 700 g of extract. Suspend the extract with about 5 times the amount of water, and extract with petroleum ether, chloroform, and n-butanol in sequence. After recovering the solvent, extracts of various fractions were obtained. Chloroform extraction part (total 190g) was chromatographed on 100-200 mesh silica gel, and petroleum ether-acetone solution with a volume ratio of 5:1 was used as the mobile phase for gradient elution to obtain 6 fractions Fr.1-Fr.6 ; Fr.2 among them was separated and purified by silica gel column, reversed phase column and gel column in turn to obtain the compound tatarinanS-T. Wherein, the silica gel H is used for the silica gel column, a...
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