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Kit for detecting carbohydrate antigen as well as detection method and application thereof

A carbohydrate antigen and kit technology, which is applied in the field of reagent kits for the detection of carbohydrate antigens, can solve the problems of low sensitivity and inaccurate detection

Active Publication Date: 2015-06-10
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the chemiluminescence immunoassay in vitro diagnostic reagents for the determination of carbohydrate antigens in conventional techniques has the problem of inaccurate or low sensitivity for samples with extremely high antigen concentrations

Method used

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  • Kit for detecting carbohydrate antigen as well as detection method and application thereof
  • Kit for detecting carbohydrate antigen as well as detection method and application thereof
  • Kit for detecting carbohydrate antigen as well as detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A kit for detecting carbohydrate antigen (CA19-9), including the following components:

[0065] 1) Magnetic microsphere system: magnetic microsphere solution coated with anti-CA19-9 monoclonal antibody 1.

[0066] Among them, the working concentration of magnetic microspheres: 0.2 mg / ml, and the working concentration of anti-CA19-9 monoclonal antibody 1: 50 μg / L.

[0067] 2) The first marker system: A solution of anti-CA19-9 monoclonal antibody 2 labeled ABEI (N-(4-aminobutyl)-N-ethylisoluminol).

[0068] Among them, the working concentration of ABEI: 1μg / L, and the working concentration of anti-CA19-9 monoclonal antibody 2: 50μg / L.

[0069] 3) The second marker system: A solution of anti-CA19-9 monoclonal antibody 2 labeled with ABEI (N-(4-aminobutyl)-N-ethylisoluminol).

[0070] Among them, the working concentration of ABEI: 1μg / L, and the working concentration of anti-CA19-9 monoclonal antibody 2: 50μg / L.

[0071] 4) Calibrator solution: CA19-9 low-point calibrator solution with...

Embodiment 2

[0098] A kit for detecting CA19-9 is basically the same as the detection kit of Example 1, except that:

[0099] 1) Magnetic microsphere system:

[0100] A solution of magnetic microspheres coated with streptavidin (SA), in which: the working concentration of magnetic microspheres: 0.2mg / ml, and the working concentration of streptavidin: 5μg / L.

[0101] Biotin labeled anti-CA19-9 monoclonal antibody 1 solution, in which: the working concentration of anti-CA19-9 monoclonal antibody 1: 50 μg / L, and the working concentration of biotin: 5 μg / L.

[0102] The preparation method of the kit for detecting CA19-9 in this example refers to the preparation method in Example 1. Except for the following steps, the rest are the same as the method in Example 1.

[0103] 1. Preparation of magnetic microsphere system.

[0104] 1. Preparation of magnetic microsphere solution coated with streptavidin (SA).

[0105] 2) Magnetic microsphere connection (magnetic microsphere connection CMC method):

[0106] The p...

Embodiment 3

[0112] A kit for detecting CA19-9 is basically the same as the detection kit of Example 1, except that:

[0113] 1) Magnetic microsphere system:

[0114] A solution of magnetic microspheres coated with goat anti-FITC (fluorescein isothiocyanate) polyclonal antibody, in which: the working concentration of magnetic microspheres: 0.2mg / ml, the working concentration of goat anti-FITC polyclonal antibody: 5μg / L.

[0115] Anti-CA19-9 monoclonal antibody 1 solution labeled with FITC, in which: the working concentration of anti-CA19-9 monoclonal antibody 1: 50 μg / L, and the working concentration of FITC: 5 μg / L.

[0116] The preparation method of the kit for detecting CA19-9 in this example refers to the preparation method in Example 1. Except for the following steps, the rest are the same as the method in Example 1.

[0117] 1. Preparation of magnetic microsphere system.

[0118] 1. Preparation of magnetic microsphere solution coated with goat anti-FITC polyclonal antibody.

[0119] 2) Magnetic ...

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Abstract

The invention discloses a kit for detecting a carbohydrate antigen as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a magnetic microsphere system: including magnetic microspheres in direct or indirect connection to an antibody 1 of the carbohydrate antigen; (2) a first marker system: including an antibody 2 in direct or indirect connection to carbohydrate antigen of a first marking tracker or a second marking tracer; and (3) a second marker system: including an antibody 2 in direct or indirect connection to carbohydrate antigen of a first marking tracker or a second marking tracer, wherein the antibody 1 of the carbohydrate antigen is different from the antibody 2 of the carbohydrate antigen in antibody site. The kit and the detection method disclosed by the invention, in detecting the carbohydrate antigen, have the advantages of being high in sensitivity and free from a HOOK effect.

Description

Technical field [0001] The present invention relates to the technical field of in vitro diagnostic detection, in particular to a kit for detecting carbohydrate antigens, and a detection method and application thereof. Background technique [0002] Tumors have posed a serious threat to human survival. The key to treating tumors lies in timely detection, early treatment and reexamination after treatment. Carbohydrate antigens are a type of tumor markers that are widely used clinically. They include glycoproteins and liposugars, often in the form of mucin. The antigenic determinants can be located on the sugar chain or on the protein core. According to the difference of glycosyl epitopes and the size of antigen epitopes, it can be generally divided into CA50, CA125, CA153, CA199, CA242, CA724. The human body's original carbohydrate antigen content is very small, or even not, but when malignant tumors occur It may induce the body to secrete high concentrations of carbohydrate antig...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/532
CPCG01N33/543G01N33/54326
Inventor 饶微李婷华徐浩明李海容胡运标袁锦云
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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