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Preparation method and culture medium of human-derived astrocyte precursor cells

A technology of astrocytes and precursor cells, applied in the field of biomedicine, can solve problems such as limited tissue sources and small number of cells, and achieve high commercial application value, simple preparation method, and high production efficiency

Active Publication Date: 2015-10-07
HUNAN SAIAOWEI BIOTECH CO LTD
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Problems solved by technology

At present, there are very few reports on human-derived astrocyte precursor cells. Most of the reports on astrocyte precursor cells are derived from rats or mice. Scientists can only isolate human brain tissue, Obtained through primary culture and identification, tissue sources are limited, and the number of cells obtained is not large

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  • Preparation method and culture medium of human-derived astrocyte precursor cells
  • Preparation method and culture medium of human-derived astrocyte precursor cells
  • Preparation method and culture medium of human-derived astrocyte precursor cells

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Embodiment Construction

[0029] The preparation method of the human-derived astrocyte precursor cells comprises the following steps:

[0030] (1) Inoculate the induced pluripotent stem cells in a 6-well cell-specific culture plate, culture the induced pluripotent stem cells at 37°C with mTeSR stem cell medium, pass passage once every 7 days, and replace 2 mL of fresh mTeSR every day stem cell culture medium;

[0031] (2) After the 5th day of induced pluripotent stem cell subculture, wash the cell surface with sterile PBS buffer, then add 1ml of embryoid body differentiation medium, and gently scrape the edge of the induced pluripotent stem cell cluster with a sterile cell scraper, The induced pluripotent stem cell clusters were separated from the culture plate and placed in a suspended state, and then repeatedly blown to disperse the induced pluripotent stem cell clusters, and the dispersed induced pluripotent stem cell clusters were transferred to a 6-well plate cell culture dish, and embryonic Shak...

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Abstract

The invention discloses a preparation method and culture medium of human-derived astrocyte precursor cells. The method can be utilized to obtain abundant astrocyte precursor cells in vitro. Compared with the traditional method of directly differentiating astrocyte cells from nerve stem cells, the precursor cells obtained by the method have high multiplication capacity and capacity for directional differentiation into astrocyte cells, and the method can quickly obtain abundant high-purity astrocyte cells in a short time. The culture medium for culturing astrocyte precursor cells has the characteristics of definite components and accessible commercial products. The method for generating abundant astrocyte cells for scientific research or clinic use has the characteristics of low cost, high safety, high production efficiency and high operability.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a method and a culture medium for preparing human astrocyte precursor cells by using induced pluripotent stem cell technology. Specifically, it relates to a method for inducing human-derived induced pluripotent stem cells (induced pluripotent stem cells, iPSCs) to differentiate into brain astrocyte progenitor cells. Background technique [0002] Astrocyte is the most widely distributed type of cell in the mammalian brain. This type of glial cell is star-shaped, with many long protrusions from the cell body. The protrusions are in contact with nerve cells to support and separate nerve cells. function. The technology of obtaining astrocytes by differentiation of induced pluripotent stem cells not only promotes the progress of neuroscience research, but also provides basic conditions for obtaining a large number of astrocytes for clinical application. Astrocytes were first obtained from h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079
Inventor 蔡娜蔡世杰
Owner HUNAN SAIAOWEI BIOTECH CO LTD
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