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Application of berberine in preparation of medicine for treating acute myeloid leukemia

A technology for acute myeloid and leukemia, applied in the field of medicine, can solve problems such as the research gap of acute myeloid leukemia, and achieve the effects of low cost, small side effects and good apoptosis rate

Active Publication Date: 2017-11-17
长沙市中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on berberine in acute myeloid leukemia caused by FLT3 mutation is almost blank

Method used

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  • Application of berberine in preparation of medicine for treating acute myeloid leukemia
  • Application of berberine in preparation of medicine for treating acute myeloid leukemia
  • Application of berberine in preparation of medicine for treating acute myeloid leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Morphological effects of berberine on the standard cell line MV-41 of refractory acute myeloid leukemia

[0025] The experimental materials used in this example are as follows: MV-41 cell line (refractory acute myeloid leukemia cells) was purchased from ATCC in the United States; Berberine (Berberine, content 98.5%) was purchased from Shaanxi Dadi Pharmaceutical Company, and the production batch number is 20111016; Cytarabine was purchased from Pfizer Pharmaceutical Co., Ltd.; DMEM medium was purchased from GIBCO; fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; NaHCO3 was purchased from Shanghai Jiuyi Chemical Reagent Co., Ltd., Cat.No.11810 -033; trypsin, EDTA, penicillin G sodium salt, and streptomycin sulfate were purchased from AMRESCO; absolute ethanol was purchased from Chongqing Chuandong Chemical Co., Ltd.; PBS was purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.; From German Leica company, the model is...

Embodiment 2

[0030] Embodiment 2, berberine to refractory acute myeloid leukemia standard cell line MV-41 half inhibitory concentration

[0031] The experimental materials used in this example are as follows: MTT (tetramethylazolazolium blue) reagent was purchased from Sigma Company; enzyme-linked immunoassay instrument was purchased from Bio-RAD Company. MV-41 cell line at 37°C, 5% CO 2 Subculture was carried out in an incubator with saturated humidity, and the cells were used for experiments when they were in the logarithmic growth phase.

[0032] Experimental method: prepare a single cell suspension with culture medium containing 10% fetal bovine serum, and use 2×10 per well 5 / mL inoculated in a 96-well culture plate with a volume of 100 μL per well, and then moved the culture plate into CO 2 in an incubator at 37 °C, 5% CO 2 and cultured for 24 h under saturated humidity conditions. Remove the culture medium, then add berberine 0.9% sodium chloride aqueous solution and cytarabine ...

Embodiment 3

[0038] Example 3. Verification of the proliferative activity of berberine on the standard cell line MV-41 of refractory acute myeloid leukemia

[0039] According to Example 2, on the basis of preliminarily determining the half-maximum inhibitory concentration and time of berberine to MV-41, the selected concentration is 50 μg / ml, 100 μg / ml, 150 μg / ml and 200 μg / ml of berberine and the concentration is 100 μg / ml, 200 μg / ml, 400μg / ml Cytarabine acted on MV-41 cells for 24h, 48h and 72h respectively, and the inhibition rate was detected. The results are shown in Table 2.

[0040] Table 2, the inhibition rate (%) n=3 of different berberine effects on MV-41 cells at different times

[0041]

[0042] As can be seen from Table 2, as the concentration of berberine increased, its inhibitory rate to cells also increased significantly. When the berberine concentration was 50 μg / ml, the 24h inhibition rate was 61.61%; when the concentration increased to 200 μg / ml , the inhibition rat...

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Abstract

The invention discloses the application of berberine in the preparation of medicine for treating acute myeloid leukemia, opens up a new use of berberine as medicine, and provides a new way for the treatment of refractory acute myeloid leukemia, and through the use of acute myeloid MV4 ‑11 cells and acute myeloid leukemia cells isolated from refractory patients were used as models, which proved that berberine has an inhibitory effect on acute myeloid MV4‑11 cells, cell cycle arrest in G1 / G2 phase, and prolonged acute myeloid leukemia mice The survival time, so berberine can be used as a new drug for the treatment of acute myeloid leukemia.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of berberine in the preparation of medicines for treating acute myeloid leukemia. Background technique [0002] Leukemia, also known as "blood cancer", is a malignant disease of hematopoietic tissues. It is characterized by malignant and unlimited proliferation of white blood cells in the bone marrow and other hematopoietic tissues, and then infiltrates various tissues and organs throughout the body, causing serious damage to the body. According to the maturity of the cells and the natural course of the disease, leukemia can be divided into two categories: acute leukemia and chronic leukemia. Acute leukemia is divided into two categories: acute lymphocytic leukemia and acute non-lymphocytic leukemia. Acute nonlymphocytic leukemia, also known as acute myeloid leukemia, is a type of disease formed by mutations in the karyotype of pluripotent stem cells or slight...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4375A61P35/02
Inventor 李杰平孙红武田志强杜方川滕永生杨励石哲
Owner 长沙市中心医院
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