Paper-based nucleic acid extraction, amplification and detection integrated detection system and paper-based nucleic acid extraction, amplification and detection integrated detection method

Abstract

The invention discloses a paper-based nucleic acid extraction, amplification and detection integrated detection system and a paper-based nucleic acid extraction, amplification and detection integrated detection method and belongs to the technical field of biological test paper detection. According to the detection method, integrated operation of nucleic acid extraction, amplification and detection of any food, environment, clinic samples and the like is achieved, and particularly, paper-based nucleic acid extraction, LAMP (loop-mediated isothermal amplification) and test paper detection are achieved. The invention further provides a small-sized handheld detection device capable of achieving nucleic acid amplification temperature. Detection results can be observed easily by naked eyes, and quantifying is achieved by mobile phone software. Total amplification and detection time is about one hour. Compared with other existing methods, the detection method has the advantages that nucleic acid extraction, amplification and detection can be simplified, operation steps are decreased and rapid efficient onsite detection is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological test paper detection, and in particular relates to a detection device and a detection method integrating paper-based nucleic acid extraction, amplification and detection. Background technique [0002] Nucleic acid detection is a research hotspot in molecular biology and has been widely used in clinical disease diagnosis and detection of epidemic bacteria or viruses. With the vigorous development of microfluidic technology, miniaturized, low-cost, and convenient detection methods, such as nucleic acid test strip detection methods, are increasingly valued. Due to the complex composition of the original sample (blood, saliva, urine, etc.) and low nucleic acid concentration, nucleic acid extraction and amplification steps are necessary before test strip detection. Routine nucleic acid extraction and amplification requires expensive equipment and is time-consuming and labor-intensive. In compariso...

AI Technical Summary

Problems solved by technology

However, nucleic acid amplification needs to be heated by an external power supply or a large heater with high power, and there are many steps. It also requires external ultraviolet light to collect detection signals, which cannot achieve efficient on-site detection.

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