A method for identification of cold resistance of grape germplasm resources during dormancy period

An identification method, the technology of mountain grapes, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve the problems of poor repeatability of measurement results, inaccurate identification results, etc., and achieve clear and followable dyeing grading boundaries, anti-corrosion Coldness is simple and easy to operate, and the results are accurate and reliable

Active Publication Date: 2018-10-23
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the determination of the cold resistance of Vitis vinifera by the current conductivity method due to the temperature, soaking time and CO in the air 2 Factors such as the dissolution of vines lead to poor repeatability of measurement results and inaccurate identification results. The present invention adopts TTC staining method to identify the cold resistance of grape germplasm resources in the dormant period.

Method used

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  • A method for identification of cold resistance of grape germplasm resources during dormancy period
  • A method for identification of cold resistance of grape germplasm resources during dormancy period
  • A method for identification of cold resistance of grape germplasm resources during dormancy period

Examples

Experimental program
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Effect test

Embodiment 1

[0048] The assay method of the dormancy period cold resistance of the grape germplasm resources provided by the present embodiment comprises the following steps:

[0049] Freezing treatment of mountain grape branches:

[0050] The grape branches of "Shuangfeng" as a germplasm resource were selected for freezing treatment. The grapevine branches in the dormant period of the germplasm resources were frozen at -25°C, -30°C, -35°C, -40°C, -45°C, and -50°C respectively. Place the vine branches at the above-mentioned 6 freezing temperatures for 10 hours, then raise the temperature to 20° C. and place them for 3 hours.

[0051] The dormant branches of Vitis vinifera were collected in mid-January from the dormant branches of the germplasm of the same maturity, cut into 20-25cm long branches, sealed with liquid paraffin, put into a sealed ziplock bag, and kept in a -15°C refrigerator save in .

[0052] Dyeing treatment of frozen branches:

[0053] Peel off the epidermis of the vine...

Embodiment 2

[0060] In the method for measuring cold resistance during the dormant period of Vitis vine germplasm resources provided in this example, the steps of freezing treatment of Vitis vine branches and dyeing treatment of frozen branches are all the same as those in Example 1.

[0061] The difference is that in the step of measuring the vigor of grape shoots, in this embodiment,

[0062] Rinse the vine branches after the above-mentioned dyeing treatment with water, cut the branches longitudinally with a blade, observe the dyed area of ​​the xylem on the longitudinal section, determine the dyeing level, and calculate the dyeing index according to the dyeing level.

Embodiment 3

[0064] The identification method of the dormancy period cold resistance of the grape germplasm resources provided by the present embodiment comprises the following steps:

[0065] Freezing treatment of mountain grape branches:

[0066] The grape branches of "Shuangfeng" as a germplasm resource were selected for freezing treatment. The grapevine branches in the dormant period of the germplasm resources were frozen at -20°C, -25°C, -30°C, -35°C, -40°C, -45°C, and -50°C respectively. Reduce the environment of the vine branches to the set freezing temperature at a cooling rate of 4 °C / h, and place them at the corresponding freezing temperature for 12 hours, then raise the temperature to 20 °C and place them for 4 hours.

[0067] The dormant branches of Vitis vinifera were collected in mid-January from the dormant branches of the germplasm of the same maturity, cut into 20-25cm long branches, sealed with liquid paraffin, put into a sealed ziplock bag, and kept in a -15°C refrigera...

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Abstract

The invention provides a method for identifying the cold resistance of grape germplasm resources in the dormant period, comprising the following steps: freezing the branches of grape grapes in the dormant period at different freezing temperatures, peeling off the skin, and putting chlorinated triphenyltetrazol In the azole staining solution, place it in a constant temperature environment for dyeing treatment; wash off the dyeing solution on the surface of the grape branch, determine the dyeing level according to the xylem dyeing area of ​​the branch of the grape, and calculate the dyeing index of the branch of the grape according to the dyeing level; according to the freezing of the branch of the grape The dyeing index corresponding to the temperature determines the low temperature half-lethal temperature. The lower the semi-lethal temperature of low temperature, the better the cold resistance of the grape germplasm. Because the TTC staining method is less affected by external factors in the determination process, and the results are intuitive, so it is simple, easy, accurate and reliable to use this method to identify the cold resistance of Vitis vine germplasm resources in the dormant period.

Description

technical field [0001] The invention relates to the field of identification of cold resistance of tree species, in particular to a method for identification of cold resistance in dormant period of grape germplasm resources. Background technique [0002] Mountain grape (Vitis amurensis Rupr.) is native to Northeast China, North Korea, and the Far East of the former Soviet Union. The natural distribution areas of Vitis vinifera in my country are mainly Changbai Mountain in Jilin Province, Wanda Mountain in Heilongjiang Province, Xiaoxing'an Mountains, mountainous areas and mid-level areas in northern Liaoning Province, and Daqing Mountain in the east of Ulanchabu League in Inner Mongolia. It is the main raw material for brewing wine in Northeast China. The wine is ruby ​​red, bright and transparent, and has a unique flavor, which is very popular among consumers. [0003] Vitis vinifera is the most cold-resistant species in the genus Vitis. The branches and vines can withstand...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/02A01G7/06A01C1/00
CPCA01C1/00A01G7/06A01G17/02
Inventor 赵滢艾军杨义明范书田王振兴王春伟何伟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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