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Evaluation method of cold resistance of germplasm resources of Vitis amurensis in dormant period

An identification method, the technology of mountain grapes, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve the problems of poor repeatability of measurement results, inaccurate identification results, etc., and achieve clear and gradable dyeing and grading boundaries. Coldness is simple and easy to operate, and the results are accurate and reliable

Active Publication Date: 2016-03-23
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the determination of the cold resistance of Vitis vinifera by the current conductivity method due to the temperature, soaking time and CO in the air 2 Factors such as the dissolution of vines lead to poor repeatability of measurement results and inaccurate identification results. The present invention adopts TTC staining method to identify the cold resistance of grape germplasm resources in the dormant period.

Method used

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  • Evaluation method of cold resistance of germplasm resources of Vitis amurensis in dormant period
  • Evaluation method of cold resistance of germplasm resources of Vitis amurensis in dormant period
  • Evaluation method of cold resistance of germplasm resources of Vitis amurensis in dormant period

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The assay method of the dormancy period cold resistance of the grape germplasm resources provided by the present embodiment comprises the following steps:

[0049] Freezing treatment of mountain grape branches:

[0050] The grape branches of "Shuangfeng" as a germplasm resource were selected for freezing treatment. The grapevine branches in the dormant period of the germplasm resources were frozen at -25°C, -30°C, -35°C, -40°C, -45°C, and -50°C respectively. Place the vine branches at the above-mentioned 6 freezing temperatures for 10 hours, then raise the temperature to 20° C. and place them for 3 hours.

[0051] The dormant branches of Vitis vinifera were collected in mid-January from the dormant branches of the germplasm of the same maturity, cut into 20-25cm long branches, sealed with liquid paraffin, put into a sealed ziplock bag, and kept in a -15°C refrigerator save in .

[0052] Dyeing treatment of frozen branches:

[0053] Peel off the epidermis of the vine...

Embodiment 2

[0060] In the method for measuring cold resistance during the dormant period of Vitis vine germplasm resources provided in this example, the steps of freezing treatment of Vitis vine branches and dyeing treatment of frozen branches are all the same as those in Example 1.

[0061] The difference is that in the step of measuring the vigor of grape shoots, in this embodiment,

[0062] Rinse the vine branches after the above-mentioned dyeing treatment with water, cut the branches longitudinally with a blade, observe the dyed area of ​​the xylem on the longitudinal section, determine the dyeing level, and calculate the dyeing index according to the dyeing level.

Embodiment 3

[0064] The identification method of the dormancy period cold resistance of the grape germplasm resources provided by the present embodiment comprises the following steps:

[0065] Freezing treatment of mountain grape branches:

[0066] The grape branches of "Shuangfeng" as a germplasm resource were selected for freezing treatment. The grapevine branches in the dormant period of the germplasm resources were frozen at -20°C, -25°C, -30°C, -35°C, -40°C, -45°C, and -50°C respectively. Reduce the environment of the vine branches to the set freezing temperature at a cooling rate of 4 °C / h, and place them at the corresponding freezing temperature for 12 hours, then raise the temperature to 20 °C and place them for 4 hours.

[0067] The dormant branches of Vitis vinifera were collected in mid-January from the dormant branches of the germplasm of the same maturity, cut into 20-25cm long branches, sealed with liquid paraffin, put into a sealed ziplock bag, and kept in a -15°C refrigera...

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Abstract

The invention provides an evaluation method of cold resistance of germplasm resources of Vitis amurensis in a dormant period. The evaluation method includes following steps: peeling a Vitis amurensis branch in the dormant period after being frozen at different freezing temperatures, placing the branch in a triphenyl tetrazoliu chloride staining solution, and disposing the solution in a constant-temperature environment for staining treatment; flushing away the staining solution on the surface of the branch, determining staining level according to staining area of a wood portion of the branch, and calculating staining index of the branch according to the staining level; determining low-temperature semi-lethal temperature according to the staining index corresponding to the freezing temperature of the branch. The lower the low-temperature semi-lethal temperature is, the higher the cold resistance of germplasms of Vitis amurensis is. A TTC staining method is adopted, thereby being slightly influenced by outside factors and visual in result, so that the evaluation method is simple, convenient and easy to implement and accurate and reliable.

Description

technical field [0001] The invention relates to the field of identification of cold resistance of tree species, in particular to a method for identification of cold resistance in dormant period of grape germplasm resources. Background technique [0002] Mountain grape (Vitisamurensis Rupr.) is native to Northeast China, North Korea, and the Far East of the former Soviet Union. The natural distribution areas of Vitis vinifera in my country are mainly Changbai Mountain in Jilin Province, Wanda Mountain in Heilongjiang Province, Xiaoxing'an Mountains, mountainous areas and mid-level areas in northern Liaoning Province, and Daqing Mountain in the east of Ulanchabu League in Inner Mongolia. It is the main raw material for brewing wine in Northeast China. The wine is ruby ​​red, bright and transparent, and has a unique flavor, which is very popular among consumers. [0003] Vitis vinifera is the most cold-resistant species in the genus Vitis. The branches and vines can withstand ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/02A01G7/06A01C1/00
CPCA01C1/00A01G7/06A01G17/02
Inventor 赵滢艾军杨义明范书田王振兴王春伟何伟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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