Infrared spectrum analysis method for identifying ABO blood types on basis of tissue slices

Abstract

The invention discloses an infrared spectrum analysis method for identifying ABO blood types on the basis of tissue slices. The method comprises the following steps of firstly collecting tissue slicesfrom human bodies with different blood types; measuring the infrared spectrum; performing spectrum data processing on the obtained spectrum by a chemometrics method; building a model for identifyingthe ABO blood types by using a mode recognition analysis method; then, taking human body tissue slices with unknown blood types; collecting the spectrum by the same method; performing spectrum data processing; and finally performing prediction by using the built model. By using the method, the ABO blood types can be accurately, durably, objectively, simply, conveniently and fast identified on thebasis of the tissue slices; the method is applicable to the tissue slices in different positions; the joint nondestructive use of the same undyed slices with pathological diagnosis can be realized; the obtained blood type identification result can provide the confirmation for blood type identification result obtained by other methods; important significance is particularly realized in forensic examination; and the blood type confirmation can be performed on broken cadavers or tissues remained in accident sites so as to fast and accurately assist the individual recognition.

Description

technical field [0001] The invention relates to a method for predicting human blood types, more specifically, an infrared spectrum analysis method for identifying ABO blood types based on tissue sections. Background technique [0002] Blood group is a genetic characteristic expressed in the form of blood antigens. The human ABO blood group system is divided into four phenotypes: A, B, AB, and O based on the presence or absence of A and B antigens on red blood cells. Type A blood has only the A antigen (A agglutinogen, which has anti-B agglutinin in its serum) on the red blood cells, and only the B antigen (B agglutinogen, which has anti-A agglutinin in the serum) on the red blood cells of type B blood. There are A and B antigens on the red blood cells of type AB blood (A and B agglutinogens, and there are no anti-A and anti-B agglutinins in the serum), and there are no A and B antigens on the red blood cells of type O blood. Antigen (there are no A and B agglutinogens, but...

AI Technical Summary

Problems solved by technology

Although existing literature (Yang Jian. Detection of ABO blood type in pathological tissue sections [J]. Chinese Journal of Forensic Medicine. 1997, 12 (4): 229-230.) has reported blood type identification based on human tissue, but its identification principle is to use red blood cells The agglutination phenomenon that is visible to the naked eye after the combination of the A or B antigen on the antibody and the corresponding antibody is identified by the red blood cell agglutination test, and the agglutination phenomenon may be affected by the reaction temperature, red blood cell or serum type, etc. Non-specific agglutination, that is, a false positive reaction , may also be affected by the ratio of reactants, reaction time, etc., resulting in insignificant agglutination, that is, a false negative reaction

In addition, the method has low sensitivity, is greatly disturbed by external factors (such as temperature), poor durability, cumbersome operation, and long detection time.

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