A kind of preparation method of lily strawberry latent ring spot virus slrsv semi-quantitative detection gold standard card

A semi-quantitative detection and ring-spot virus technology, applied in measurement devices, instruments, scientific instruments, etc., to achieve the effect of strong detection, good repeatability and intuitive results

Active Publication Date: 2017-10-10
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0009] However, at present, the prevention and control of lily virus is mainly based on killing the transmission medium - aphids. As for how to scientifically spray anti-lily virus agents, whether the proliferation of lily virus is inhibited after spraying anti-viral agents, and if so, what is the effect of the inhibition? There are no relevant reports on the extent of the problem, and the solution to these problems first depends on semi-quantitative detection; therefore, the realization of semi-quantitative detection is of great significance

Method used

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  • A kind of preparation method of lily strawberry latent ring spot virus slrsv semi-quantitative detection gold standard card
  • A kind of preparation method of lily strawberry latent ring spot virus slrsv semi-quantitative detection gold standard card

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Embodiment Construction

[0024] Such as figure 1 with figure 2 The shown gold standard card for semi-quantitative detection of SLRSV includes a gold standard card slot 1, a backing plate 12, a sample pad 8, a colloidal gold binding pad 9, a nitrocellulose membrane 10, and a water-absorbing filter paper 11, wherein the gold standard card slot 1 includes the upper The casing and the lower casing, the upper casing and the lower casing are connected by buckles, the upper casing is provided with a sample injection hole 2 and a reaction window 3, the sample pad 8 is placed under the sample injection hole 2, and the nitrocellulose membrane 10 is placed Below the reaction window 3, the gold standard probe is contained on the colloidal gold binding pad 9, the backing plate 12 is fixed in the gold standard card slot 1, the sample pad 8, the colloidal gold binding pad 9, the nitrocellulose membrane 10 and the water-absorbing filter paper 11 are sequentially Arranged and connected to the upper surface of the l...

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Abstract

The invention discloses a semi-quantitative detection gold labeling card for a lily strawberry latent ringspot virus and a preparation method. For improving the usage efficiency and increasing the field popularizing rate of a colloidal gold immunochromatography detection method and achieving quick and semi-quantitative detection on the lily virus, the number of detection lines is increased to three, a known quantity of antibodies with the concentration difference are wrapped with the detection lines, the gold labeling card capable of achieving quick and semi-quantitative detection on the SLRSV is developed, and the requirements for large-scale virus eradication of lilies and quick and semi-quantitative detection on the lily virus in a field are met. The gold labeling card is high in detection pertinence, easy and convenient to operate, high in accuracy and sensitivity and capable of accurately detecting the SLRSV content difference among samples without the help of any instrument and equipment.

Description

technical field [0001] The invention relates to a method for preparing a gold standard card for rapid semi-quantitative detection of lily virus, specifically referring to a method for preparing a gold standard card capable of rapid and semi-quantitative detection of lily strawberry latent ring spot virus SLRSV by using colloidal gold immunochromatography . Background technique [0002] lily( Lilium spp. ) is the Liliaceae ( Liliaceae ) Lilium ( Lilium ) is a perennial monocotyledonous herbaceous plant. It is a world-renowned bulb flower with a long history of cultivation. It integrates ornamental, edible and medicinal uses. As the largest flower planting country in the world, the Netherlands has a relatively mature lily cut flower and bulb industry. ; In China, the edible and medicinal lily industry has developed well. The main production areas are in Gansu, Hunan and Hubei. The main varieties are Lanzhou lily, Longya lily (wild lily), etc.; With the rapid increase of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 张玉宝陈延王亚军谢忠奎王若愚王乐郭志鸿
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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