Diclazuril colloidal gold test paper strip preparation method and usage method thereof

A technology of colloidal gold test paper and diclazuril, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection cost, unfavorable promotion and application, and complexity.

Inactive Publication Date: 2016-04-13
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In food and feed, the detection methods of diclazuril include thin-layer chromatography, gas chromatography, gas-mass coupling and high-performance liquid chromatography. Since the sample pretreatmen...

Method used

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  • Diclazuril colloidal gold test paper strip preparation method and usage method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Detection of diclazuril in chicken

[0031] Pre-treatment: take 2g of chicken sample, add 20ml of sample extract (dilute methanol with deionized water at a volume ratio of 9:1, that is, 9 parts of methanol plus 1 part of deionized water, used to extract diclazuril in the sample ); shake vigorously for 5 minutes; centrifuge at 4000r / min for 5 minutes, or filter with ordinary filter paper; dilute the supernatant or filtrate with deionized water at a ratio of 1:19; take the diluted liquid for testing.

[0032] Diclazuril semi-quantitative rapid detection test strip product usage method: Take 100 μg of the sample filtrate and slowly drop it on the glass fiber membrane of the test strip, and observe the detection line (T line) and reference line on the test strip after 15 minutes (Line C) color rendering. Determine the content of diclazuril in the sample extract according to the detection principle of the test strip: if only one red color band appears, it is judge...

Embodiment 2

[0033] Example 2 Detection of diclazuril in feed

[0034]Pre-treatment: Take 2g of pulverized sample, add 20mL of sample extraction solution (dilute methanol with deionized water at a volume ratio of 9:1, that is, 9 parts of methanol plus 1 part of deionized water, used to extract diclazuril in the sample ); shake vigorously for 5 minutes; centrifuge at 4000r / min for 5 minutes, or filter with ordinary filter paper; dilute the supernatant or filtrate with deionized water at a ratio of 1:49; take the diluted liquid for testing.

[0035] Diclazuril semi-quantitative rapid detection test strip product usage method: Take 100 μL of the sample filtrate and slowly drop it on the glass fiber membrane of the test strip, and observe the detection line (T line) and reference line on the test strip after 15 minutes (Line C) color rendering. Determine the content of diclazuril in the sample extract according to the detection principle of the test strip: if only one red color band appears, ...

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Abstract

The invention belongs to the field of biological detection, and relates to a diclazuril colloidal gold test paper strip preparation method and a usage method thereof. The test paper strip comprises a paperboard, an absorbent pad, a detection pad, a colloidal gold pad and a sample pad are sequentially pasted on one side of the paperboard from up to down, and adjacent pads overlappingly connect at a joint; the detection pad treats a cellulose nitrate membrane as a base pad, a transverse reference wire and a detection wire are arranged on the cellulose nitrate membrane in a top-down manner, the detection wire is coated with a diclazuril-bovine serum albumin conjugate, and the reference wire is coated with a rabbit-anti-mouse polyclonal antibody; and the colloidal gold pad is transversely sprayed with a nanogold labeled anti-diclazuril monoclonal antibody which is generated by a hybridoma cell strain. The test paper strip which is used for diclazuril detection has the characteristics of rapid detection, simple operation and high sensitivity.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a method for detecting residual substances in food, in particular to a method for preparing and using diclazuril colloidal gold test strips. Background technique [0002] Diclazuril Diclazuril belongs to the triazine benzyl nitrile compound, which is a new type, high-efficiency, low-toxic anticoccidial drug, widely used in chicken coccidiosis. The peak period of the main action on coccidia varies with different species of coccidia, such as the main action point on Eimeria tenella is in the sexual cycle of the second generation schizont coccidia. But the giant, Eimeria brucei schizont invalid. The point of action on Eimeria maxima is at the zygote stage of Eimeria brucei; it is highly effective on the microgametophyte stage of Eimeria brucei. Diclozolid also inhibited the formation of sporulated oocysts. In food and feed, the detection methods of diclazuril include thin-lay...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 洪霞江振飞
Owner JIANGSU WISE SCI & TECH DEV
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