Preparation method and application of immunochromatographic test strip for detecting caffeine residues

Abstract

The invention relates to a test paper for detecting excessive caffeine, and a preparation method thereof. According to the method, a sample liquid absorption part, a colloidal gold labeling part, a detection reaction part and a water absorption part are sequentially adhered to a lining of the test paper. The detection reaction part is coated with an antigen for detection as a detection line, and is also coated with an IgG1 strip for resisting second species animal protein as a reference line. The rapid detection test strip is strong in specificity, can realize semi-quantitative detection, andis suitable for rapid detection of excessive caffeine in canned beverage production plants, food hygiene quality inspection departments, personal families and the like. The test strip is used for detecting caffeine, and has the characteristics of rapid detection, simplicity in operation and high sensitivity.

Description

technical field [0001] The invention belongs to the field of food safety testing, and specifically relates to a detection method for substances exceeding the standard in food, in particular a caffeine test paper detection method. Background technique [0002] Caffeine is an alkaloid extracted from tea leaves and coffee cherries. Appropriate use can relieve fatigue and excite nerves. It is clinically used to treat neurasthenia and coma recovery. However, high-dose or long-term use will also cause damage to the human body, especially it is also addictive. Once it is stopped, various withdrawal symptoms such as mental depression, fatigue and weakness will appear. Symptoms are not very serious. However, due to drug tolerance, when the dosage of the drug continues to increase, caffeine will not only act on the cerebral cortex, but also directly excite the medulla oblongata, causing paroxysmal convulsions and bone tremors, and damage important internal organs such as the liver, s...

AI Technical Summary

Problems solved by technology

However, high-dose or long-term use will also cause damage to the human body, especially it is also addictive. Once it is stopped, various withdrawal symptoms such as mental depression, fatigue and weakness will appear. Symptoms are not very serious. However, due to drug tolerance, when the dosage of the drug continues to increase, caffeine will not only act on the cerebral cortex, but also directly excite the medulla oblongata, causing paroxysmal convulsions and bone tremors, and damage important internal organs such as the liver, stomach, and kidneys. Induces diseases such as respiratory inflammation, women's breast tumors, and even leads to mental retardation and limb deformities in the next generation of smokers

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