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Preparation and using method of 2-aminobutane colloidal gold test strip

A technology of colloidal gold test paper and colloidal gold paper sheet, which can be used in measurement devices, instruments, scientific instruments and other directions, and can solve problems such as unfavorable popularization and application, high cost, etc.

Inactive Publication Date: 2016-02-03
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the detection of sec-butylamine mainly uses gas phase and liquid chromatography analysis. These methods not only need expensive instruments and equipment, but also have relatively high requirements for the detection materials, and need further purification before they can be carried out, which is not conducive to popularization and application.

Method used

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  • Preparation and using method of 2-aminobutane colloidal gold test strip
  • Preparation and using method of 2-aminobutane colloidal gold test strip
  • Preparation and using method of 2-aminobutane colloidal gold test strip

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Embodiment Construction

[0029] The sec-butylamine detection card is equipped with a detection test strip 2 in the detection card shell 1. The structure of the detection card shell 1 and the setting of the detection test strip 2 are shown in the appendix. figure 1 , the detection card shell 1 is a long, flat, thin shell, with a length of 70mm, a width of 20mm, a thickness of 5mm, and a thin shell wall thickness of 1mm, which is made of engineering plastics. , There are detection window holes 3 and sample injection holes 4 on the cover of the detection card shell 1 . The test strip 2 is placed in the lower cover of the test card casing 1 .

[0030] The detection test strip 2 is a multi-layered narrow strip with a length of 60 mm, a width of about 4 mm and a thickness of less than 2.5 mm. The detection test strip 2 is a multi-layer structure: the bottom layer is a supporting back plate 5, which is a polyethylene sheet, with a thickness of about 0.5 mm, a length of 60 mm, and a width of 4 mm; 0.5mm, le...

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Abstract

The invention discloses preparation and a using method of a quick detection test strip of 2-aminobutane. The test strip provided by the invention sequentially comprises filter sample paper, immune colloidal gold paper, a nitrocellulose membrane and absorbent paper from the sample adding end, wherein the immune colloidal gold paper is a glass fiber gasket coated with an anti-2-aminobutane monoclonal antibody labeled by colloidal gold; the immune nitrocellulose membrane is a nitrocellulose membrane provided with a detection line and a quality control line; the nitrocellulose membrane is coated with a strip of detection-use antigen used as the detection line, and is coated with a strip of second species animal protein resistant IgG (intravenous gamma globulin) used as a reference line. The 2-aminobutane colloidal gold test strip has the benefits of being high in specificity and sensitivity, simple and convenient to operate, and the like.

Description

technical field [0001] The invention belongs to the field of food safety detection, and particularly relates to a preparation and use method of a rapid colloidal gold test strip for sec-butylamine drug residues in food. Background technique [0002] Sec-butylamine (2-aminobutane, 2-AB for short), also known as 2-aminobutane, is a protective fungicide that has antibacterial activity against a variety of fungi and can be used as a preservative during storage for garlic sprouts, green peppers, citrus, and grapes. preserver. 2-AB was first widely used in Europe, and it has been used in my country since 1976. There are no reports of carcinogenic and mutagenic effects of 2-AB at home and abroad, however. The United States has set its hazard level to human health as three. The scope of application and the maximum allowable residues are stipulated. The residues of litchi, citrus and apple (pulp) are ≤0.009g / kg, 0.005g / kg and 0.001g / kg respectively. [0003] Too much sec-butylami...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 杜道林江振飞洪霞
Owner JIANGSU WISE SCI & TECH DEV
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