Experimental animal model with non-alcoholic steatohepatitis transforming into hepatic carcinoma

Abstract

The present invention relates to the field of biotechnology and in particular to a construction method of an experimental animal model, specifically a mouse model, with non-alcoholic steatohepatitis (NASH) transforming into hepatic carcinoma (HCC), and use thereof. The present invention provides a construction method of a mouse model with NASH and chronic steatohepatitis developing into chronic hepatic fibrosis, cirrhosis and HCC. The construction method comprises the following steps: newborn C57BL / 6 mice (including male and female newborn mice) are subjected to subcutaneous single injection of streptozotocin (STZ) (100-400 [mu]g / newborn mouse) and the mice begin to be fed with experiment use high-fat feed after the completion of female mouse breastfeeding to construct the NASH mouse model with hepatic fibrosis, cirrhosis and HCC. The provided mouse model is relatively simple in construction process, relatively simple in technical means, and low in construction cost, has an extremely high value as the needed animal model in scientific research and drug research and development, and belongs to a domestic initiative.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for constructing a large and mouse model of an experimental animal model in which non-alcoholic steatohepatitis transforms into liver cancer and its application. The animal model constructed by the present invention can be used for chronic fatty liver, steatohepatitis, liver fibrosis, liver cirrhosis, hepatocellular carcinoma, metabolic diseases, diabetes, in vivo experiments of related mechanisms of diabetic complications, new drug research and development, in non- It has extremely high application value in the research and drug development of the pathogenesis of alcoholic steatohepatitis (NASH), liver fibrosis and liver cancer. Background technique

[0002] Existing animal and mouse models for NASH, liver fibrosis, liver cirrhosis, and liver cancer animal experiments are divided into chemical poison-induced mouse models, physical injury mouse models, and pathological diet-...

Examples

Embodiment Construction

[0007] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0008] Taking C57BL / 6 mice as an example, a method for making an animal model of non-alcoholic steatohepatitis transformed into liver cancer is provided, including the steps of:

[0009] (1) C57BL / 6 mice were bred and pregnant.

[0010] (2) Subcutaneous injection of STZ (200 micrograms per animal) on the back of offspring 24 hours after birth.

[0011] (3) The lactation period of offspring is 4 weeks. The mother mice were fed with normal standard feed during lactation.

[0012] (4) After the end of the 4-week lact...

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for constructing a large and mouse model of an experimental animal model in which non-alcoholic steatohepatitis transforms into liver cancer and its application. The animal model constructed by the present invention can be used for chronic fatty liver, steatohepatitis, liver fibrosis, liver cirrhosis, hepatocellular carcinoma, metabolic diseases, diabetes, in vivo experiments of related mechanisms of diabetic complications, new drug research and development, in non- It has extremely high application value in the research and drug development of the pathogenesis of alcoholic steatohepatitis (NASH), liver fibrosis and liver cancer. Background technique

[0002] Existing animal and mouse models for NASH, liver fibrosis, liver cirrhosis, and liver cancer animal experiments are divided into chemical poison-induced mouse models, physical injury mouse models, and pathological diet-...

Examples

Embodiment Construction

[0007] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0008] Taking C57BL / 6 mice as an example, a method for making an animal model of non-alcoholic steatohepatitis transformed into liver cancer is provided, including the steps of:

[0009] (1) C57BL / 6 mice were bred and pregnant.

[0010] (2) Subcutaneous injection of STZ (200 micrograms per animal) on the back of offspring 24 hours after birth.

[0011] (3) The lactation period of offspring is 4 weeks. The mother mice were fed with normal standard feed during lactation.

[0012] (4) After the end of the 4-week lact...