Colorectal cancer prognosis markers, expression evaluation methods, kits and applications

A colorectal cancer and prognosis judgment technology, applied in the biological field, can solve problems such as tumor recurrence and achieve the effect of convenient detection

Inactive Publication Date: 2019-05-10
SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in many patients, the tumor may recur even if the TNM stage is early

Method used

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  • Colorectal cancer prognosis markers, expression evaluation methods, kits and applications
  • Colorectal cancer prognosis markers, expression evaluation methods, kits and applications
  • Colorectal cancer prognosis markers, expression evaluation methods, kits and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 SLC12A5 up-regulation test in colorectal cancer

[0035] The immunohistochemical method of the embodiment of the present invention was used to detect the expression level of SLC12A5 in colorectal cancer and normal intestinal mucosa respectively, and it was found that SLC12A5 was silent in normal intestinal mucosa, but was up-regulated in colorectal cancer. The result is figure 1 As shown, figure 1 This is the result of the expression level of SLC12A5 in Example 1 of the present invention in colorectal cancer and normal intestinal mucosa. Among them, picture (a) shows colorectal cancer tissues (CRC tumor tissues), picture (b) shows normal tissues adjacent to cancer (Adjacentnormal tissues).

[0036] Based on the up-regulation of SLC12A5 in colorectal cancer, 15 pairs of colorectal cancer tissue samples were selected, and the expression of SLC12A5 was analyzed by immunohistochemistry. The result is figure 2 As shown, figure 2 This is the relative expression result...

Embodiment 2

[0037] Example 2 In vivo test of the effect of knocking down the expression of SLC12A5 on tumor growth in nude mice

[0038] The control cell lines HCT116 / control or HCT116 / shSLC12A5 were randomly injected into the back of nude mice, and the tumor growth patterns were compared. The tumor volume is measured every 3 days for approximately three weeks. Calculate the tumor volume (mm) by measuring the longest and shortest tumor diameter length (volume formula = 0.5 × length × 2 × width) 3 ). 22 days after the inoculation, the experiment was terminated and the mice were sacrificed. The tumor growth curve of nude mice is like Figure 4 As shown, where C represents the tumor growth curve injected with HCT116 / control, and S represents the tumor growth curve injected with HCT116 / shSLC12A5. The average tumor size of nude mice injected with HCT116 / shSLC12A5 was significantly lower than that of control group nude mice injected with HCT116 / control (P Figure 5 As shown, the histogram repres...

Embodiment 3

[0059] Example 3 Evaluation of the expression level of SLC12A5 in colorectal cancer tissue

[0060] In Example 3, the colorectal cancer prognostic judgment marker expression evaluation method of the present invention was used to evaluate the expression level of SLC12A5 in colorectal cancer tissue. The method for detecting the expression level of SLC12A5 in this method is an immunohistochemical method. The specific evaluation process is as follows:

[0061] S10: Obtain 195 cases of colorectal cancer wax specimens.

[0062] S20: Cut the embedded wax block into 5 micron thick sections.

[0063] S30: Perform immunohistochemical detection on the slice. The specific process of this step is shown in steps S31 to S37 described above.

[0064] S40: Evaluate the expression level of colorectal cancer markers based on the percentage of SLC12A5 positive cell staining. Using the scoring standard of the present invention, the level of SLC12A5 was detected by immunohistochemistry on tissue samples ...

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Abstract

The invention discloses a colorectal cancer prognosis marker. The amino acid sequence of the colorectal cancer prognosis marker (SLC12A5) is represented by SEQ No.1. The invention also discloses an application of the colorectal cancer prognosis marker in the preparation of kits, an expression evaluation method of the colorectal cancer prognosis marker, a kit, and applications of the kit. The high-level expression of colorectal cancer prognosis marker (SLC12A5) and bad prognosis of patients form a relationship of positive correlation, and thus the prognosis situation of patients can be judged according to the expression level of SLC12A5. Through the expression evaluation method, the expression level of SLC12A5 can be judged. The SLC12A5 can be used to prepare a kit, which is used to detect the expression level of colorectal cancer prognosis marker, and thus the expression level of SLC12A5 can be detected conveniently.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and in particular relates to a colorectal cancer prognostic judgment marker, the application of the marker in the preparation of a kit, a method for evaluating the expression of the marker, a kit containing the marker, and the kit in Used in the detection of the expression level of markers for colorectal cancer prognosis. Background technique [0002] Colorectal cancer, including colon cancer and rectal cancer, is a common malignant tumor worldwide. Its fatality rate ranks second in the global cause of death from malignant tumors, and the prognosis of advanced colorectal cancer is poor. According to the data released by the International Organization for Research on Cancer in 2005, the incidence of colorectal cancer is on the rise, most of which are colon cancer. The standard incidence rate of colon cancer in our country is 131 / 100,000, and the incidence rate is increasing obviously. The occurre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574C07K14/82
Inventor 于君沈祖尧许丽霞
Owner SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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