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A kind of method for in vitro regeneration of Chinese tallow adventitious root

A technology of in vitro regeneration and tallow tree, applied in the field of plant biology, to achieve the effect of convenient material collection and simple regeneration process

Active Publication Date: 2018-04-20
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report yet about utilizing the adventitious roots of aseptic seedlings of superior strains as explants to regenerate Chinese tallow tree plants

Method used

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  • A kind of method for in vitro regeneration of Chinese tallow adventitious root
  • A kind of method for in vitro regeneration of Chinese tallow adventitious root
  • A kind of method for in vitro regeneration of Chinese tallow adventitious root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for in vitro regeneration of Chinese tallow adventitious roots, the specific operations are as follows:

[0037] 1. Disinfect and cultivate the stem segments with axillary buds of the excellent strains of Chinese tallow tree in the field to obtain aseptic seedlings of Chinese tallow tree with strong root system;

[0038] 2. Cut the adventitious roots of the aseptic seedlings obtained in the first step as explants, cut the adventitious roots into sections of about 0.3 cm, and inoculate them in the cluster bud induction medium, at a temperature of 24 °C and a light intensity of 2000 lx. Under the condition that the photoperiod is 14 / 10h (light / dark), the induction of clustered buds is carried out, wherein the clustered bud induction medium is MS+0.1mg / L 6-BA+0.01mg / L NAA+0.01mg / L IBA+30g / L sucrose+7.5g / L agar, pH=5.8;

[0039] 3. After 1-2 weeks of light culture, clustered buds are induced at both ends of adventitious roots, the induction rate of clustered buds ...

Embodiment 2

[0043] A method for in vitro regeneration of Chinese tallow adventitious roots, the specific operations are as follows:

[0044] 1. Disinfect and cultivate the stem segments with axillary buds of the excellent strains of Chinese tallow tree in the field to obtain aseptic seedlings of Chinese tallow tree with strong root system;

[0045] 2. Cut the adventitious roots of the aseptic seedlings obtained in the first step as explants, cut the adventitious roots into sections of about 0.4 cm, and inoculate them in the cluster bud induction medium at a temperature of 25°C and a light intensity of 2500 lx. Under the condition that the photoperiod is 14 / 10h, the cluster buds are induced ( figure 1 ), wherein the cluster bud induction medium is MS+3.5mg / L 6-BA+1.5mg / L NAA+0.75mg / L IBA+30g / L sucrose+7.5g / L agar, pH=5.8;

[0046] 3. After 1 to 2 weeks of light culture, clustered shoots were induced at both ends of adventitious roots and around them ( figure 2 ), the induction rate of clu...

Embodiment 3

[0050] A method for in vitro regeneration of Chinese tallow adventitious roots, the specific operations are as follows:

[0051] 1. Disinfect and cultivate the stem segments with axillary buds of the excellent strains of Chinese tallow tree in the field to obtain aseptic seedlings of Chinese tallow tree with strong root system;

[0052] 2. Cut the adventitious roots of the aseptic seedlings obtained in the first step as explants, cut the adventitious roots into sections of about 0.6 cm, and inoculate them in the cluster bud induction medium at a temperature of 26 °C and a light intensity of 3000 lx. Under the condition that the photoperiod is 14 / 10h, the clustered buds are induced, wherein the clustered bud induction medium is MS+8.0mg / L 6-BA+3.0mg / LNAA+1.0mg / L IBA+30g / L sucrose +7.5g / L agar, pH=5.8;

[0053] 3. After 1-2 weeks of light culture, clustered buds were induced at both ends of adventitious roots and around them, the induction rate of clustered buds was 83.4%, and ...

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Abstract

The invention discloses an in vitro regeneration method of adventitious roots of sapium sebiferum roxb. The in vitro regeneration method comprises the following steps: using adventitious roots of sterile seedlings obtained through in vitro rapid propagation of sapium sebiferum roxb strains as explant materials, and cutting the explant materials into segments; inoculating a plurality of adventitious root cut segments into inducing culture mediums, and performing induction of multiple shoots or induction of callus tissues so as to obtain multiple shoots or callus tissues; switching adventitious root explants of the produced multiple shoots or adventitious root explants of the produced callus tissues into propagation culture, and performing proliferation of the multiple shoots or the proliferation of the callus tissues; cutting the proliferated callus tissues into dices, switching the callus tissue dices into differentiation culture mediums, and performing differentiation; switching the proliferated multiple shoots and the callus tissues differentiating the multiple shoots into multiple shoot elongation culture mediums, and performing the elongation of the multiple shoots; after the multiple shoots elongate, switching the elongated multiple shoots into rooting culture mediums, and performing rooting; after the adventitious roots are induced from the sterile seedlings, performing domestication and transplanting so as to obtain complete sapium sebiferum roxb regeneration plants. The in vitro regeneration method disclosed by the invention has the advantages that the germplasm characteristics of pistillate plants are maintained, materials are convenient to take, no pollution is generated, and the rapid propagation of good single plants of wild sapium sebiferum roxb can be realized.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for in vitro regeneration of Chinese tallow adventitious roots. Background technique [0002] Chinese tallow tree (Sapium sebiferum Roxb.), together with tung tree, camellia oleifera, and walnut, is listed as the four major woody oil plants in my country. It is a deciduous tree of the genus Sapium in the family Euphorbiaceae, a native oil tree species in China, and has a cultivation history of 1,400 years in China. In addition, Chinese tallow tree has a beautiful posture and beautiful leaf shape. After autumn, the leaves show red, orange, yellow, purple and other colors. It integrates fruit viewing, leaf viewing, and shape viewing. subtropical region. Sapium seeds contain both oil and fat. The extracted oil is divided into tallow and catalpa oil. Sallow tallow is a traditional export substance and an important industrial raw material in my country. It i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯金艳吴丽芳赵华毛颖基陈雪
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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