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Cultivation method for regeneration of sapindus mukorossi gaertn. strain

A cultivation method and technology of Sapindus japonica, applied in the field of plant biology, can solve the problems of cumbersome regeneration process, poor repeatability and low regeneration efficiency, and achieve the effects of convenient material collection, fast regeneration speed and high regeneration efficiency.

Active Publication Date: 2015-06-03
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
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Problems solved by technology

At present, the research on the tissue culture of Sapindus is mainly focused on the use of stems or leaves as explants to induce buds or callus, and there are generally problems such as low regeneration efficiency, cumbersome regeneration process, and poor repeatability.

Method used

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  • Cultivation method for regeneration of sapindus mukorossi gaertn. strain

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Embodiment Construction

[0029] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0030] preferred embodiment

[0031] A kind of cultivation method of Sapindus strain regeneration, see figure 1 , including the following steps:

[0032] (1), strain selection: select Sapindus petiole; the preferred Sapindus petiole is taken from the year-old petiole of the perennial Sapindus excellent strain in the field. In this way, on the one hand, the germplasm characteristics of the female parent plant are maintained; on the other hand, the material is convenient and abundant; and the rapid propagation of the excellent single plant of Sapindus in the wild is realized, and the variety cultivation is realized...

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Abstract

The invention discloses a cultivation method for regeneration of a sapindus mukorossi gaertn. strain. The cultivation method for the regeneration of the sapindus mukorossi gaertn. strain comprises the following steps: selecting plants, disinfecting, sectioning, carrying out embryoid induction culture, embryoid proliferation and subculture, embryoid maturation culture and embryoid mature seedling culture, and transplanting aseptic seedlings. Compared with the prior art, the cultivation method for the regeneration of the sapindus mukorossi gaertn. strain has the advantages that regeneration efficiency of embryoids cultivated by adopting the cultivation method for the regeneration of the sapindus mukorossi gaertn. strain is high, and induction rate reaches up to 92.5%; embryoid regeneration rate is high, the propagation coefficient is high, hundreds of and even ten hundreds of embryoids are produced after each explant is cultured for 15-17 weeks on the average, and the excellent characteristics of a sapindus mukorossi gaertn. female plant can be maintained, so that important technical support is provided for rapid propagation and genetic improvement of an excellent sapindus mukorossi gaertn. strain.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a breeding method for regeneration of Sapindus strains. Background technique [0002] Sapindus mukorossi Gaertn., also known as Sapindus mukorossi Gaertn., is a deciduous tree of Sapindus family Sapindus. All-in-one all-purpose tree species. The pure natural washing products made of its peel are very popular in many countries and regions. It not only has strong detergency, but also has antibacterial, anti-inflammatory, and anti-oxidant effects. It is an ideal raw material for biodiesel; in addition, Sapindus has a beautiful shape and is full of golden colors in autumn, so it is also called golden tree, which is an excellent landscaping tree species. Regardless of whether it is a garden tree species or an economic tree species, its cultivation prospects are very broad, and the market demand is large, but the inefficient seedling breeding technology is the bottleneck restrictin...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吴丽芳侯金艳沈羊城刘文博赵薇薇穆燕吴影毛颖基
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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