A method for improving stem section regeneration efficiency of excellent strains of Chinese tallow tree in the field

Abstract

The invention discloses a method for improving the regeneration efficiency of stem segments of excellent strains of Chinese tallow tree in the field. The current year's stem segments of excellent strains of Chinese tallow tree in the wild are taken as explants, and are subjected to disinfection, induction, multiplication, elongation, rooting and transplanting, etc. Six steps to obtain robust Chinese tallow tree regenerated plants. Compared with the prior art, the present invention has the following advantages: it can maintain the germplasm characteristics of the female parent plant, it is convenient to obtain materials, and the materials are abundant, and it can realize rapid propagation of excellent single plants of Chinese tallow tree in the field, and realize variety cultivation; the disinfection procedure is simple and the disinfection time is short , less toxic to explants, low pollution rate, high regeneration efficiency of clustered buds, fast regeneration speed, high reproduction coefficient, suitable for all genotypes of Chinese tallow tree, and has broad application prospects.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for improving the stem segment regeneration efficiency of excellent strains of Chinese tallow tree in the field. Background technique [0002] Sapium sebiferium (Sapium sebiferium), also known as wax tree and tallow tree, is a perennial deciduous tree of the genus Sapium in Euphorbiaceae. . The oil content of Chinese tallow tree seeds is as high as 55%. It is an ideal raw material for biodiesel production and is a unique economic tree species for oil in China. Accompanied by strong resistance to cold, drought, waterlogging and salt-alkali, it is an excellent landscaping tree species and has a cultivation history of 1,400 years in China. Therefore, Chinese tallow tree has important economic and ecological value whether it is used as an economic crop for oil or as a tree species for landscaping. With the accelerated development of biomass energy forestry and landscape...

AI Technical Summary

Problems solved by technology

At present, researches on the tissue culture of Chinese tallow tree mainly focus on using the endosperm, leaves, hypocotyls or roots of seedlings as explants to induce buds or callus, or using young Chinese tallow tree embryos as explants to pass through the body. The cell embryogenesis pathway induces the regeneration of Chinese tallow tree plants, however, due to the highly heterozygous genetic background characteristics of Chinese tallow tree, the above-mentioned techniques are not suitable for the preservation and propagation of excellent mother plants (germplasm) of Chinese tallow tree

In recent years, there have also been reports on the rapid propagation of Chinese tallow tree by using the stem section of the wild Chinese tallow tree with axillary buds as explants, but there are generally problems such as high pollution rate, serious browning, low reproduction coefficient, and poor repeatability, which are difficult to meet. The need for large-scale rapid propagation and variety improvement of Chinese tallow tree excellent strains