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Method for extracting astaxanthin from haematococcus pluvialis

A technology of Haematococcus pluvialis and Haematococcus pluvialis powder, applied in the direction of organic chemistry and the like, can solve the problems of difficult mass production and high cost

Inactive Publication Date: 2016-08-17
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the process of industrialization, the cost may be too high, and it is not easy to produce on a large scale.

Method used

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Experimental program
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Effect test

Embodiment Construction

[0016] The specific method for extracting astaxanthin from Haematococcus pluvialis is as follows:

[0017] (1) Dissolve 1g of Haematococcus pluvialis powder in 40mL of 4M HCl with an analytical balance, and bathe in water at 80°C for 5 minutes;

[0018] (2) After acid treatment, centrifuge at 7000rpm for 5min, pour out the supernatant, wash twice with deionized water, dissolve the bottom precipitate with 40mL DMSO for 5min, and take a sample as a;

[0019] (3) Ultrasound for 30 minutes in an ice bath, and the relevant setting parameters are 30 minutes of total working time, 1 second of work, 1 second of rest, and 390 W of power. Sampling after ultrasound is recorded as b;

[0020] (4) After ultrasonication, take a sample in a water bath at 45°C for 1 hour and record it as c. Samples a, b, and c were passed through a 0.22 μm organic filter membrane, and the content of astaxanthin was determined by liquid chromatography. Then calculate the extraction rate of astaxanthin.

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Abstract

The invention discloses a method for extracting astaxanthin from haematococcus pluvialis. The method has the advantages that simple acid treatment serving as a haematococcus pluvialis cell wall breaking method is combined with a fat-soluble solvent dimethyl sulfoxide freely soluble to the astaxanthin, and factor optimization of the acid treatment wall breaking method arrives at a conclusion that when concentration of hydrochloric acid is 4 M and a ratio of haematococcus pluvialis powder to the hydrochloric acid is 1 to 40 (w / v) and treatment is conducted for 5 minutes at a temperature lower than 80 DEG C, the dimethyl sulfoxide is adopted as the extracting solvent to reach the maximum extracting rate ranging from 0.2% to 0.5%; in order to increase the extracting rate of the astaxanthin, the acid treatment wall breaking method is combined with a ultrasonic wall breaking method to achieve better effect, and the extracting rate of the astaxanthin is up to 0.4%-0.7%.

Description

technical field [0001] The invention belongs to microalgae biotechnology, and relates to a method for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Haematococcus pluvialis, a ubiquitous green alga belonging to Volvox, Haematococcus family, is one of the natural sources for the production of astaxanthin. Astaxanthin, as a xanthophyll in carotenoids, has extremely important applications in fish coloration and human body anti-oxidation. [0003] Haematococcus pluvialis can accumulate a large amount of astaxanthin during the period of thick-walled spores, which is generally the best time to collect Haematococcus pluvialis. The cell wall of the chlamydospore stage is thicker, and the effect of breaking the wall is very important for the extraction of astaxanthin, and the practical factors such as the economic cost of the method and industrialized large-scale production must also be considered comprehensively. In recent years, with the contin...

Claims

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Application Information

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IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 冯嵬朱明辉
Owner BEIJING UNIV OF CHEM TECH