In-situ detection method of honeysuckle flower tannin

An in-situ detection and honeysuckle technology, which is applied in the direction of measuring devices, test plant materials, and preparation of test samples, can solve the problems of inability to display the position of tannins and in-situ analysis, and achieve the effect of saving time

Inactive Publication Date: 2016-09-07
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The characteristic of the past detection method is to extract tannins from honeysuckle first, and then detect them with an absorbance photometer according to the light absorption characteristics of tannins. This method is conducive to quantitative analysis, but it cannot be analyzed in situ, because it cannot show that tannins are in honeysuckle. s position

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The in-situ detection method of tannin in this example honeysuckle fresh sample comprises the following steps:

[0019] In the first step, the different tissues of flower buds are cut into small pieces or slices (less than 1.0 mm × 1.0 mm) with double-sided blades, soaked in 1 ml of 2% glutaraldehyde solution (prepared with pH 6.8 phosphate buffer) for 2 Hours, the container is a 1.5 ml capped centrifuge tube, and the temperature is room temperature. During the period, use a vacuum pump to pump air;

[0020] In the second step, suck out the glutaraldehyde solution in the tube, soak it with pH 6.8 phosphate buffer solution for 3 times, each time for 20 minutes, then add 0.3 ml of 1% osmium tetroxide aqueous solution, and soak the sample in it for 2 hours , the temperature is room temperature;

[0021] The third step is to suck out the osmium tetroxide solution and dehydrate it with a gradient of 10% alcohol series, starting from 30% alcohol until 100% alcohol, 1 ml per...

Embodiment 2

[0026] The in-situ detection method of tannin in the honeysuckle dried sample of this example comprises the following steps:

[0027] In the first step, the dried flower buds are fully hydrated in phosphate buffer solution, and different tissues are cut into small pieces or small pieces (less than 1.0 mm × 1.0 mm) with a double-sided blade, and mixed in 1 ml of 2% glutaraldehyde solution (with a pH of 6.8 Prepared in phosphate buffer solution) for 2 hours, the container is a 1.5 ml centrifuge tube with a cover, and the temperature is room temperature. During the period, use a vacuum pump to pump air;

[0028] In the second step, suck out the glutaraldehyde solution in the tube, soak it with pH 6.8 phosphate buffer solution for 3 times, each time for 20 minutes, then add 0.3 ml of 1% osmium tetroxide aqueous solution, and soak the sample in it for 2 hours , the temperature is room temperature;

[0029] The third step is to suck out the osmium tetroxide solution and dehydrate ...

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PUM

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Abstract

The invention provides an in-situ detection method of honeysuckle flower tannin. The method comprises the following steps: (1) a honeysuckle flower sample is immersed in a 2% glutaraldehyde solution for 12 hours; (2) extra glutaraldehyde in the sample is cleaned, and the sample is immersed in a 1% osmium tetroxide aqueous solution for 4 hours; (4) the sample is dehydrated by alcohol with gradient, and each alcohol gradient is used for 15 minutes; (4) the dehydrated sample is embedded in resin; (5) the sample is sliced, and the thickness is 1-2 micrometers; (6) tannin is observed by microscopic observation.

Description

technical field [0001] The invention relates to an in-situ detection method of honeysuckle tannin. Background technique [0002] Honeysuckle is not only used as a traditional Chinese medicine, but also used in the production of health drinks. Tannins affect the taste of beverages, so it is necessary to detect tannins in honeysuckle. The characteristic of the past detection method is to extract tannins from honeysuckle first, and then detect them with an absorbance photometer according to the light absorption characteristics of tannins. This method is conducive to quantitative analysis, but it cannot be analyzed in situ, because it cannot show that tannins are in honeysuckle. s position. Contents of the invention [0003] Based on the problems existing in the background technology, the present invention proposes a kind of in-situ detection method of honeysuckle tannin, comprising the following steps: [0004] 1, a kind of in-situ detection method of honeysuckle tannin, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/28G01N1/36
CPCG01N21/84G01N1/28G01N1/36G01N2021/8466
Inventor 刘林廉玉姬赵小梅
Owner LINYI UNIVERSITY
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