Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunochromatography kit for bone marker detection and preparation method thereof

An immunochromatography and marker technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of lack of sensitivity and timeliness in the evaluation of osteoporosis treatment effects, and achieve high detection sensitivity, simple operation, and ease of use. control effect

Active Publication Date: 2018-02-16
BEIJING MOKOBIO LIFE SCI CO LTD +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

BMD is a good method for diagnosing osteoporosis, but it lacks sufficient sensitivity and timeliness for fracture risk prediction and evaluation of osteoporosis treatment effect, which has certain limitations.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunochromatography kit for bone marker detection and preparation method thereof
  • Immunochromatography kit for bone marker detection and preparation method thereof
  • Immunochromatography kit for bone marker detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The kit for detection of bone markers in this embodiment includes a plastic plate, a glass cellulose membrane set on the plastic plate, a nitrocellulose membrane set on the glass cellulose membrane, and a sample is arranged at one end of the nitrocellulose membrane. The corresponding other end is provided with a water-absorbing pad, and the glass cellulose membrane is coated with quantum dot-labeled type I collagen carboxy-terminal peptide (CTX) antibody at a mass ratio of 1:1:1, quantum dot-labeled bone A mixture of calcein (OC) antibody and quantum dot-labeled type I procollagen amino-terminal propeptide (PINP) antibody; the nitrocellulose membrane is provided with a detection line 1, a detection line 2, a detection line 3 and a control line, The detection line 1 is coated with type I collagen carboxy-terminal peptide (CTX) secondary antibody, the detection line 2 is coated with osteocalcin (OC) secondary antibody, and the detection line 3 is coated with type I collage...

Embodiment 2

[0058] The kit for detecting bone markers in this example differs from the example in that the quantum dots used are cadmium sulfide quantum dots, the modified cyclodextrin used is aminated dry cyclodextrin, and the type I collagen carboxyl In the process of peptide-terminal (CTX) antibody, quantum dot-labeled osteocalcin (OC) antibody and quantum dot-labeled type Ⅰ procollagen N-terminal propeptide (PINP) antibody, the activated quantum dot-modified cyclodextrin -The mixing time for adding the antibody or antigen to the latex suspension is 2 hours, and the others are the same as in Example 1.

Embodiment 3

[0060] The kit for detection of bone markers in this example differs from the example in that the type I collagen carboxy-terminal peptide (CTX) antibody, the quantum dot-labeled osteocalcin (OC) antibody and the quantum dot-labeled type I protoplast During the process of the collagen amino-terminal propeptide (PINP) antibody, the mixing time of adding the antibody or antigen to the activated quantum dot-modified cyclodextrin-latex suspension was 4 hours, and the others were the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of immunochromatography detection, in particular to an immunochromatography kit for bone marker detection and a preparation method of the immunochromatography kit. The kit comprises a plastic plate, a glass cellulose membrane, a nitrocellulose membrane, a sample pad and a water absorbing pad, wherein the glass cellulose membrane is coated with a mixture of a quantum dot marked type I collagen carboxy-terminal telopeptide antibody, a quantum dot marked osteocalcin antibody and a quantum dot marked procollagen type I N-terminal propeptide antibody; a detection line 1, a detection line 2 and a detection line 3 are arranged on the nitrocellulose membrane; the detection line 1 is coated with a type I collagen carboxy-terminal telopeptide second antibody, the detection line 2 is coated with an osteocalcin second antibody, and the detection line 3 is coated with a procollagen type I N-terminal propeptide second antibody. The kit realizes rapid and sensitive joint detection of a type I collagen carboxy-terminal telopeptide, osteocalcin and a procollagen type I N-terminal propeptide with a fluorescence immunochromatography method through the double antibody / antigen sandwich principle in combination with the fluorescence characteristic of quantum dots.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to an immunochromatography kit for bone marker detection and a preparation method thereof. Background technique [0002] Osteoporosis is a systemic bone disease characterized by low bone mass and damage to bone microarchitecture, resulting in increased bone fragility and susceptibility to fracture. In 2001, the American NIH expert group added a decrease in bone strength to the definition of osteoporosis. Osteoporosis is divided into three categories. The first category is primary osteoporosis, which is a physiological degenerative disease that inevitably occurs with age, accounting for more than 90% of all osteoporosis; the second category is primary osteoporosis. The second category is secondary osteoporosis, which is induced by some factors such as other diseases or drugs; the third category is idiopathic osteoporosis, which is more common in adolescents or...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 金鑫曾滨赵正道
Owner BEIJING MOKOBIO LIFE SCI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com