A device for detecting and isolating circulating melanoma cells

Abstract

The invention relates to a device for detecting and separating circulating melanoma cells, including a computer control system, a photoelectric detection system electrically connected to the computer control system, and a blood flow circulation unit used in conjunction with the photoelectric detection system. The system includes a pulse laser, a cylindrical lens sequentially arranged between the pulse laser and the blood circulation unit, a mechanical slit, a convex lens, a reflector, an objective lens and an ultrasonic probe opposite to the objective lens, and the ultrasonic probe is electrically connected with a computer control system. Compared with the prior art, the present invention has simple overall structure, convenient operation, intelligent control, high detection accuracy, high minimally invasive safety, economical and practical, and can achieve circulating tumor detection without mixing markers into the blood. Cell detection and separation of trace blood containing circulating melanoma cells can be widely used in the fields of clinical medicine and basic medical research.

Description

technical field [0001] The invention belongs to the technical field of cell detection and relates to a cell detection and separation device, in particular to a device for detection and separation of circulating melanoma cells. Background technique [0002] Melanoma is a highly malignant tumor, most of which originate in the skin, eyes, nasal cavity and other parts, and can metastasize to lungs, brain and other important parts in the early stage of tumor formation. Circulating tumor cells (English abbreviation: CTC) refer to cells separated from the primary tumor and entering the circulatory system, with the ability to attach to other parts of the body and form metastatic lesions. Circulating melanoma cells are an important indicator of melanoma metastasis. The number of circulating melanoma cells is of great significance for judging the process of tumor metastasis. Timely removal of circulating tumor cells will help to inhibit the early metastasis process of melanoma and red...

AI Technical Summary

Problems solved by technology

First of all, it lacks the implementation details of the sheath flow system necessary for the formation of single-cell liquid flow, so it is not feasible; secondly, the patent splits the already very mature fluorescence-based flow cytometry technology, and all related fluorescent labeling and detection The content has been published; the detection range of 1Hz-100MHz in ultrasonic testing has obviously expanded the detection range intentionally, and the patent has not published the detection results, and the implementation of the technical solution still requires complex processing of blood or other Yang Ping single cell flow

[0006] Therefore, regardless of the above-mentioned methods, it is necessary to mix artificially synthesized materials into the blood to label circulating tumor cells or perform complex processing on blood samples, resulting in a slow detection speed, which largely limits its application to the living state. Circulating Tumor Cell Isolation under

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