A Strain of Aspergillus tubingensis ct1 and Its Application in Solubilizing Phosphorus in Saline-alkali Land

A technology of Aspergillus tabin and Aspergillus bini, which is applied in the fields of biotechnology and agricultural yield increase, can solve problems such as low yield and low phosphorus utilization efficiency, and achieve the effects of improving utilization rate and promoting crop yield increase.

Active Publication Date: 2018-02-23
BINZHOU UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of low crop yield and low phosphorus utilization efficiency in saline-alkali land, the present invention provides a strain of Aspergillus tubingensis CT1 and its application in saline-alkali land depletion of phosphorus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Strain of Aspergillus tubingensis ct1 and Its Application in Solubilizing Phosphorus in Saline-alkali Land
  • A Strain of Aspergillus tubingensis ct1 and Its Application in Solubilizing Phosphorus in Saline-alkali Land
  • A Strain of Aspergillus tubingensis ct1 and Its Application in Solubilizing Phosphorus in Saline-alkali Land

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 strain screening

[0022] The Yellow River Delta was collected, and the soil samples were screened according to the cultivation method of Martin's medium, and the colonies with large transparent circles, high transparency, and vigorous growth were selected for isolation, screening and purification, until a single colony was isolated and then placed for observation. Store on the slant of Shi's medium in a refrigerator at 4°C. We obtained 14 strains of phosphate-solubilizing bacteria through primary screening.

[0023] The 14 strains of phosphate-solubilizing bacteria were re-screened using inorganic phosphorus medium, and the phosphate-solubilizing bacteria with the strongest phosphorus-solubilizing ability were selected to measure the phosphorus-solubilizing ability of organic phosphorus. Finally, the phosphate-solubilizing bacterium Aspergillus tubingensis CT1 was obtained, which had a high phosphorus-solubilizing ability, up to 523.5 mg / L. The result is as...

Embodiment 2

[0025] Embodiment 2: Morphological characteristics of Aspergillus tubingensis bacterial strain

[0026] Put a piece of filter paper at the bottom of the petri dish, put a glass slide rack on the filter paper, put a glass slide and two coverslips on the glass slide, cover the dish, use newspaper for external use, seal it, and extinguish it by high-pressure steam Sterilize at 0.101MPa and 121°C for 20 minutes in a bacteria pot, and dry in an oven at low temperature. Use a sterilized dropper to draw a small amount of melted Chapei culture medium, drop 1 / 2 drop to the inoculation site of the glass slide, and then use an inoculation needle to pick a small amount of mycelium of Phospholyticum to be observed to the slide glass in the wet chamber a. Before the culture medium is completely solidified, cover the cover glass so that the distance between the cover glass and the glass slide is quite close but not in contact. Finally, pour an appropriate amount of 20% sterile glycerin int...

Embodiment 3

[0029] Example 3 Sequence determination and identification of Aspergillus tubingensis strain

[0030] ITS sequence analysis uses PDA solid medium to cultivate fungi, scrape the mycelium on the surface of the plate, put it into a mortar and grind it with liquid nitrogen. Genomic DNA was extracted from the ground mycelia using a fungal total DNA extraction kit. PCR amplification was carried out by using the PCR universal primers of the fungal ITS sequence (the upstream primer is ITS1: 5'-TCCGTAGGTGAACCTGCGG-3'; the downstream primer is ITS4: 5'-TCCTCCGCTTATTGATATGC-3'). A 25 μl PCR reaction system was used: 2.5 μmol L-1 dNTPs, 10 μmol L-1 upstream primer, 10 μmol L-1 downstream primer, 3U Taq plus DNA polymerase, 1×PCR reaction buffer. The PCR amplification program was: 94°C for 5 min; 94°C for 1 min, 57°C for 45 s, 72°C for 45 s, a total of 35 cycles; 72°C for 10 min.

[0031] The PCR amplification product was detected by agarose gel electrophoresis with a mass fraction of 1....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a strain of Aspergillus tubingensis CT1 and its application in saline-alkaline soil phosphate solution. The preservation number of Aspergillus tubingensis CT1 is: CGMCC No.12770; the depository unit is: General Microbiology Center of China Microbiological Culture Collection Management Committee; The preservation date is: July 20, 2016; the preservation address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The invention can effectively improve the utilization rate of soil phosphorus, and further promote crop yield increase.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and agricultural production increase, in particular, it relates to a kind of Aspergillus tubingensis CT1 and its application in saline-alkali soil phosphorus solution. Background technique [0002] Saline-alkali land is widely distributed in my country, especially in the Yellow River Delta region, which makes agricultural benefits low and crop yield low. Phosphorus is one of the essential nutrients for plants, but most of the phosphorus in the soil exists in the inorganic or organic state that is difficult for plants to use. The difficult-to-use phosphorus accounts for 95% of the total phosphorus in the soil, which greatly reduces the utilization efficiency of phosphorus by plants. [0003] Phosphorus solubilizing bacteria can improve the utilization of soil phosphorus. Phosphorus solubilizing bacteria produce various enzymes such as phytase, nuclease, phospholipase and organic acids in the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/66C09K101/00
CPCC09K17/14C09K2101/00C12N1/145C12R2001/66
Inventor 李学平谢文军荣琨
Owner BINZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap