A method and system for individual identification and paternity testing of unknown pig samples

A technology of paternity identification and material inspection, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., and can solve the problems of poor cumulative individual recognition rate, poor genetic polymorphism of loci, serious problems, etc.

Active Publication Date: 2019-11-19
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned sites are all dinucleotide repeat sequences, the stutter peaks in the typing results are relatively serious, and the genetic polymorphism of some sites is poor, and there is a problem of poor cumulative individual recognition rate

Method used

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  • A method and system for individual identification and paternity testing of unknown pig samples
  • A method and system for individual identification and paternity testing of unknown pig samples
  • A method and system for individual identification and paternity testing of unknown pig samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1. Verification of the accuracy of the method and system for individual identification and paternity identification of unknown pig samples of the present invention

[0048] In this example, the unknown pig samples are 100 pig anticoagulant blood samples (53 males and 47 females), whose individual sources are known, but their individual sources are set during the implementation of Example 1 of the present application. The source is unknown, and the method and system of this application are used for individual identification and paternity identification, including:

[0049] 1) Using the DNA extraction system in the system of the present invention to extract DNA from unknown pig samples, 2) Using the composite detection system in the system of the present invention to obtain the DNA including 12 autosomal STR loci and sex-determining loci PigSTRAM 3) use the deduction system described in the system of the present invention to carry out individual identification and p...

Embodiment 2

[0076] Embodiment 2 Analysis of cumulative individual recognition rate and cumulative non-parent exclusion rate of the detection system of the present invention

[0077] Using the system of the present invention to obtain the typing results of 240 unrelated individuals of pigs according to the method described in Example 1, and calculate the individual recognition rate, heterozygosity, and polymorphism of Chinese pigs for 12 autosomal STR loci in these results Information content, non-parent exclusion rate, and match probability.

[0078] Specifically, Powerstats v1.2 software was used to calculate individual recognition rate (DP), heterozygosity (H), polymorphic information content (PIC), non-parent exclusion rate (PE), and matching probability (Pm), as shown in Table 6 below . And the cumulative non-parent exclusion rate and cumulative individual discrimination were calculated according to the following formulas 1-2.

[0079] Table 6

[0080] DP H PIC PE ...

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Abstract

The invention provides a method and system for performing individual identification and paternity identification on an unknown pig subject. The method comprises the following steps: extracting DNA of an unknown pig subject, wherein the DNA comprises typing results of 13 gene loci (12 euchromosome STR gene loci and sex determination gene locus PigSTRAM), and the 12 euchromosome STR gene loci are SW240, PigSTR13E, PigSTR 15A, S0655, PigSTR 11B, PigSTR 14A, 387A12F, PigSTR 4C, PigSTR 17A, PigSTR 5C, PigSTR 7B and S0386; and carrying out individual identification and paternity identification according to the genotypes of the 13 gene loci of the unknown pig subject. The invention also provides a system for performing individual identification and paternity identification on an unknown pig subject. The scheme provided by the invention can implement individual identification and paternity identification on an unknown pig subject.

Description

technical field [0001] The invention relates to a method and system for individual identification and paternity identification, in particular to a method and system for individual identification and paternity identification of unknown pig samples. Background technique [0002] Microsatellite markers (microsatellite), also known as short tandem repeats (short tandem repeats, STRs) or simple sequence repeats (simple sequence repeats), refer to repeating sequences with a repeating unit length of 2-6 bases. STRs are currently the most commonly used class of genetic markers in forensic DNA analysis due to the high inter-individual variability and abundance of repeat units. [0003] In addition to DNA analysis of humans, forensic DNA analysis technology can also be used for species identification, individual identification and paternity identification of animals. Pigs are common domestic animals in rural areas of our country. With the deepening of people's understanding of patern...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 马温华张颖孙启凡莫晓婷张建白雪叶健赵兴春
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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