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A method for preparing β-carotene microcapsules

A technology of carotene and microcapsules, which is applied in food forming, food ingredients as encapsulants, food science, etc., to reduce production costs, low cost, and good water solubility

Active Publication Date: 2018-09-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no relevant report on the direct utilization of γ-CGTase modified starch products from the Bacillus sp. Related reports of purification directly used for embedding in microcapsules

Method used

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  • A method for preparing β-carotene microcapsules
  • A method for preparing β-carotene microcapsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Take cassava starch as substrate, add deionized water to heat and gelatinize, make a solution with a concentration of 1% (w / v), cool to room temperature, take 1mL of substrate, add 2U / g CGTase, and place at constant temperature The reaction was carried out on a vibrating mixer for 24 hours, and after the end, the enzyme was killed in a boiling water bath for 10 minutes. Then centrifuge at 5000r / min for 10min, dry in a freeze dryer for 48h, collect the samples, grind them slightly and store them in a ziplock bag at 4°C for refrigerator storage.

[0022] (2) Weigh the enzymatic hydrolyzate and β-carotene according to the mass ratio of 1:1, then add water to dissolve, so that the solid content is 25%, fill with nitrogen protection, 200W ultrasonic 10min, place in a vibrating mixer for 800r / min, react at 50°C for 1 hour, cool down, and freeze-dry to obtain microcapsules.

[0023] Adopt the method for gel chromatography to measure molecular weight after CGTase catalyze...

Embodiment 2

[0026] (1) Take cassava starch as substrate, add deionized water to heat and gelatinize, make a solution with a concentration of 2% (w / v), cool to room temperature, take 1mL of substrate, add 8U / g CGTase, and place at constant temperature The reaction was carried out on a vibrating mixer for 24 hours, and after the end, the enzyme was killed in a boiling water bath for 10 minutes. Then centrifuge at 5000r / min for 10min, dry in a freeze dryer for 48h, collect the samples, grind them slightly and store them in a ziplock bag at 4°C for refrigerator storage.

[0027] (2) Weigh the enzymatic hydrolyzate and β-carotene according to the mass ratio of 2:1, then add water to dissolve, so that the solid content is 25%, fill with nitrogen protection, 200W ultrasonic 10min, place in a vibrating mixer for 800r / min, react at 50°C for 5h, cool down, and freeze-dry to obtain microcapsules.

Embodiment 3

[0029] (1) Take cassava starch as substrate, add deionized water to heat and gelatinize, make a solution with a concentration of 4% (w / v), cool to room temperature, take 1mL of substrate, add 4U / g CGTase, and place at constant temperature The reaction was carried out on a vibrating mixer for 24 hours, and after the end, the enzyme was killed in a boiling water bath for 10 minutes. Then centrifuge at 5000r / min for 10min, dry in a freeze dryer for 48h, collect the samples, grind them slightly and store them in a ziplock bag at 4°C for refrigerator storage.

[0030] (2) Weigh the enzymatic hydrolyzate and β-carotene according to the mass ratio of 4:1, then add water to dissolve, so that the solid content is 25%, fill with nitrogen protection, 200W ultrasonic 10min, place in a vibrating mixer for 800r / min, react at 50°C for 3 hours, cool down, and freeze-dry to obtain microcapsules.

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Abstract

The invention discloses a method for preparing a beta-carotene microcapsule from a product of CGTase (cyclodextrin glucosyltransferase) catalyzed starch. Gamma-CGTase of Alkalophilic Bacillus sp. G-825-6 is used for catalyzing starch under the conditions that, preferably, the starch concentration is 4%, the enzyme dose is 4 U / g and the reaction is performed at 50 DEG C for 24 h, and the catalyzed product is directly used for embedding beta-carotene under the embedding conditions that the subject-object mass ratio is 4: 1, the reaction temperature is 50 DEG C and the reaction time is 3 h. The product has the embedding rate of 51% for beta-carotene under the optimal embedding condition and has the beta-cyclodextrin embedding effect equivalent to that reported in literatures. The method is simple and easy to implement, the water solubility of the CGTase modified product is more than 20 times that of beta-cyclodextrin, the product has high beta-cyclodextrin solubilizing multiple and wider application range, and the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of microcapsule preparation, and in particular relates to a method for preparing beta-carotene microcapsules by using gamma-CGTase catalyzed starch products as wall materials. Background technique [0002] It is reported that cyclodextrin has the properties of hydrophobic inside the ring and hydrophilic outside the ring, which can embed hydrophobic substances and play a stabilizing role. As a good wall material, it is more and more widely used in the technology of microcapsules field. Cyclodextrin is mostly derived from the enzymatic hydrolysis of starch, that is, through cyclodextrin glucosyltransferase (CGTase), the starch is converted into cyclodextrin. At present, most of the products of CGTase acting on starch are α, β, γ three A mixture of cyclodextrins. However, the efficiency of CGTase catalyzed starch is usually not high, generally less than 50%, that is, 50% of the non-cyclic components are separ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23P10/30A23L29/30
CPCA23V2002/00A23V2200/224A23V2250/211A23V2250/5114A23V2250/5112
Inventor 王金鹏金征宇田耀旗徐学明周星柏玉香王萍赵建伟谢正军焦爱权
Owner JIANGNAN UNIV