Method for detecting monocytelike myeloid-derived suppressor cells in human peripheral blood

Abstract

The invention relates to a method for detecting monocytelike myeloid-derived suppressor cells in human peripheral blood. Used antibodies comprise an antibody APC anti-human CD33 (a), an antibody PE anti-human CD15 (b), an antibody PE-CF594 anti-human HLA-DR (c), an antibody APC-H7 anti-human CD14 (d), an antibody PE-Cy7 anti-human CD45 (e), and an antibody FITC anti-mouse CD11b (f). A flow type sample solution is prepared; the ratio of mo-MDSCs in the flow type sample solution is detected by a flow cytometer. For the detection of mo-MDSCs, the clear number screening range and specific screening range are given. The method has the advantages that the detection is simple, the operation is convenient, and the detection time is short; only a trace amount of peripheral blood sample markers is required, the cost is low, and the method is suitable for being widely popularized; the peripheral blood samples of 151 testing persons are respectively detected by the method; the detected mo-MDSCs ratio values are obviously different in different populations, and the ratio in a breast cancer patient is obviously higher than the ratio in a healthy person and a breast benign tumor patient; the detection results are calculated and analyzed by Graphpad Prism 6 software via a computer, P is smaller than 0.05, the statistic meaning is realized, and the results are scientific and credible.

Description

technical field [0001] The invention relates to a method for detecting mononuclear myeloid-derived suppressor cells in human peripheral blood. Background technique [0002] Myeloid-derived suppressor cells (MDSCs) are a group of heterogeneous cells derived from bone marrow, which can be divided into two subgroups according to their morphology and surface markers: granulocytic myeloid-derived suppressor cells (PMN-MDSCs) and Mononuclear myeloid-derived suppressor cells (mo-MDSCs). Among them, mo-MDSCs have strong immunosuppressive ability 1 , mo-MDSCs present in the body can affect the progression and exacerbation of breast cancer and other diseases. Based on this, the screening and detection of mo-MDSCs is very necessary. [0003] Due to the lack of specific identification markers for MDSCs cell population, the existing screening and detection methods are very mixed. ALMAND B et al. considered that Lin − HLA-DR − Can be used as a marker to screen and detect MDSCs cell ...

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Problems solved by technology

However, the existing detection methods for MDSCs and mo-MDSCs mostly rely on the operators with rich exp

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