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Micro-column gel card agglutination test result recognition method

A micro-column gel card and detection result technology, which is applied in the field of medical devices, can solve the problems of affecting analysis results, misjudgment, incomplete reaction area, etc., and achieve the effect of improving accuracy and improving versatility

Active Publication Date: 2018-10-16
SUZHOU HYBIOME BIOMEDICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned method of directly intercepting the image of the reaction area of ​​a fixed size in the microtube for analysis is only applicable to a certain size of the microcolumn gel card. When the width of the microcolumn gel card used is different, the intercepted reaction area It is not comprehensive, or the interception area is too large, which may affect the final analysis results and cause misjudgment.

Method used

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  • Micro-column gel card agglutination test result recognition method
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  • Micro-column gel card agglutination test result recognition method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Such as figure 1 As shown, it is a flow chart of the microcolumn gel card agglutination detection result identification method of the present invention, and the method includes the following steps:

[0042] (1) Collect images, collect images of micro-column gel cards that have undergone agglutination and centrifugation;

[0043] (2) Intercepting the microtube image in the microcolumn gel card image;

[0044] (3) Obtain the target area to be identified in the microtubules image, the target area is the image area within the microtube inner wall;

[0045] (4) In the above-mentioned target domain, capture the effective reactant area, and identify the position and relative area of ​​the captured effective reactant area;

[0046] (5) Judging and outputting the agglutination detection result of the microtubules according to the preset standards of reactant position and relative area.

[0047] Such as Figure 4 As shown, the method for intercepting the microtubules image in...

Embodiment 2

[0055] On the basis of Example 1, the effective reactant area is captured and identified from the above-mentioned target area, and the judgment and identification is carried out according to the preset standard. The effective reactant area appears on the image as the bright area A and the sub-bright area B in the above target area image, such as image 3 , 7 and 8 are shown. In the step (4), the effective reactant region is captured, and the method for identifying the position and relative area of ​​the captured effective reactant region comprises the following steps: B1, searching for a bright region, and identifying the position of the bright region in the microtube; B2, finding For the sub-bright area, the position and relative size of the sub-bright area in the microtubules are identified. Among them, the bright area is the image area where the gray value in the target area is greater than or equal to the first threshold, that is, the area where red blood cells in the mi...

Embodiment 3

[0075] Such as Figure 9 In the shown embodiment 3, on the basis of the embodiment 1 or 2, a step of result confirmation is added, and the agglutination detection result output above is manually confirmed to complete the identification of the microtubule agglutination detection result. For example, after the result is judged, the judgment result is output in the form of a pop-up window or other forms, and manual confirmation of the result is required, and the system ends the recognition work only after confirmation. After manual confirmation, misjudgment due to instrument failure or other reasons is avoided.

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Abstract

The present invention relates to the technical field of medical instruments. Disclosed is a method of identifying a micro-column gel card agglutination test result, comprising the following steps: collecting an image of a micro-column gel card, capturing an image of a micro-tube, acquiring a target region, capturing and identifying an effective reactant region, and finally determining and outputting an identification result. A boundary of an inner wall of a micro-tube is automatically fit by means of a binary processing, and a reaction region within the boundary of the inner wall of the micro-tube is acquired to be a target region for analysis, thus preventing a portion of the image outside the region from interfering with the analysis and determination, effectively increasing the accuracy of determination, thereby enabling the method to be applied to a variety of micro-column gel cards having different specifications and improving the universality thereof for analyzing systems of different specifications. The accuracy of result identification is further increased by performing a classification determination, based on both a brightness position and relative area, on the effective reactant region in the target region under analysis, and then performing a comparative analysis of a coarse classification and a fine classification.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and relates to a method for identifying agglutination detection results of a microcolumn gel card. Background technique [0002] At present, clinical blood testing generally adopts the cassette detection method with simple operation, clear results and stability. To control the size of the gel gap, so that the gap can only allow free red blood cells to pass through, so that free red blood cells and aggregated red blood cells are separated. The detection principle is: when the antigen and antibody react, the blood cells agglutinate, and when centrifuged, the agglutination cannot pass through the gap of the gel, but stays in the upper layer of the gel tube, and the reaction is positive. Unagglutinated blood cells can pass through the gel gap during centrifugation and settle at the bottom of the gel tube, giving a negative reaction. [0003] In an automated blood analyzer, the judgment of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
CPCG01N35/00
Inventor 吴锋闫晓磊沙利烽
Owner SUZHOU HYBIOME BIOMEDICAL ENG CO LTD
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