Unfreezing device for placental cells capable of indicating recovering end point and recovering method thereof

Abstract

The invention discloses a placental cell thawing device capable of indicating the end point of resuscitation and a resuscitation method thereof, consisting of a water bath and a resuscitation cell device arranged in the water bath; the resuscitation cell device is provided with a resuscitation box, a clean water tank and a control unit. Panel, the control panel is equipped with a power switch, a filter control switch, a temperature indicator light, a temperature control device and an alarm device, and the filter control switch controls the operation of the water pump; the top of the resuscitation box is provided with a resuscitation fixing plate, and the surface of the resuscitation fixing plate is provided with fixing holes , the shape and diameter of the fixing holes correspond to the shape and size of the cryopreservation tube and the cryopreservation bag respectively; the lower surface of the resuscitation fixing plate is provided with elastic clamping pieces at positions corresponding to the fixing holes. With the device and method, there is no need to manually hold the cryopreservation bag or the cryopreservation tube during the resuscitation process, which realizes automation; overcomes the defect that manual visual inspection is required to check whether the resuscitation is complete, and at the same time overcomes the problem that the water in the water bath is easy to cause pollution; improves The consistency of recovery temperature improves cell viability.

Description

technical field [0001] The present invention relates, specifically, to a placental cell thawing device and a resuscitating method that can indicate the end point of resuscitating. Background technique [0002] Patent CN204151347 discloses a cell resuscitator with water bath circulation. In this invention, the cryopreservation tube placement hole is set as a locking hole, and the cryopreservation tube is fixed in the water bath, and it has the effect of isolating the water in the resuscitator. However, there are still the following disadvantages: 1. The end point of cell recovery needs to be manually judged; 2. Although the cell cryopreservation tube is not in direct contact with water, the risk of introducing pollution is avoided, but the specific heat of air is much smaller than that of water, which will greatly slow down the process. The heating speed of the cryopreservation tube does not meet the technical requirements of "instant dissolution" for cell recovery, which may...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a placental cell thawing device and its resuscitation method that can indicate the end point of resuscitation, which can be applied to the resuscitation of cryopreservation tubes and cryopreservation bags. , no need to manually hold the cryopreservation bag or cryopreservation tube, which realizes automation; overcomes the defect that manual visual inspection is required to check whether the resuscitation is complete, and at the same time overcomes the problem that the water in the water bath is easy to cause pollution; improves the consistency of the resuscitation temperature and improves cell activity

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