Unfreezing device for placental cells capable of indicating recovering end point and recovering method thereof

Abstract

The invention discloses an unfreezing device for placental cells capable of indicating a recovering end point and a recovering method thereof. The device comprises a water bath kettle and a cell-recovering device arranged in the water bath kettle, wherein the cell-recovering device is equipped with a recovering box, a water purifying box and a control panel; the control panel is equipped with a power switch, a filtering control switch, a temperature indicator, a temperature control device and an alarming device; the filtering control switch is used for controlling a water pump to work; a recovering fixed plate is arranged at the top of the recovering box; a fixing hole is formed on the surface of the recovering fixed plate; the hole shape and the hole diameter of the fixing hole are respectively corresponding to the shape and size of a cryopreserving pipe and a cryopreserving bag; and an elastic clamping sheet is arranged on the position, corresponding to the fixing hole, on the lower surface of the recovering fixed plate. According to the device and the method, a user does not need to hold the cryopreserving pipe or the cryopreserving bag in the recovering process and the automation is realized; the defect of manual visual inspection required for checking if completing the recovering is overcome; the pollution problem easily caused by water in water bath can be solved; and the recovering temperature consistency is promoted and the cell activity is promoted.

Description

technical field [0001] The present invention relates, specifically, to a placental cell thawing device and a resuscitating method that can indicate the end point of resuscitating. Background technique [0002] Patent CN204151347 discloses a cell resuscitator with water bath circulation. In this invention, the cryopreservation tube placement hole is set as a locking hole, and the cryopreservation tube is fixed in the water bath, and it has the effect of isolating the water in the resuscitator. However, there are still the following disadvantages: 1. The end point of cell recovery needs to be manually judged; 2. Although the cell cryopreservation tube is not in direct contact with water, the risk of introducing pollution is avoided, but the specific heat of air is much smaller than that of water, which will greatly slow down the process. The heating speed of the cryopreservation tube does not meet the technical requirements of "instant dissolution" for cell recovery, which may...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a placental cell thawing device and its resuscitation method that can indicate the end point of resuscitation, which can be applied to the resuscitation of cryopreservation tubes and cryopreservation bags. , no need to manually hold the cryopreservation bag or cryopreservation tube, which realizes automation; overcomes the defect that manual visual inspection is required to check whether the resuscitation is complete, and at the same time overcomes the problem that the water in the water bath is easy to cause pollution; improves the consistency of the resuscitation temperature and improves cell activity

Images