A method for constructing an animal with atherosclerotic liver depression syndrome
A technology of atherosclerosis and construction method, which can be used in animal feed, drug equipment, additional food elements, etc., can solve problems such as inability to give affirmation, liver cell damage, and threat to human healthy life.
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Embodiment 1
[0039] This embodiment provides a method for constructing atherosclerotic liver depression syndrome mice, which includes the following steps:
[0040] S1) The experimental mice were all fed with high-fat diet (fat mass percentage content 21%+cholesterol mass percentage content 0.15%), and the left common carotid artery cannulation (constricted silicone tube) was performed on the mice at the beginning of the experiment. To induce common carotid atherosclerotic lesions, construct mice with AS plaques; the experimental process of mice follows the animal ethics norms;
[0041] S2) 8 weeks after the animal carotid cannulation operation, on the first day, the mice were kept in a breeding cage inclined at 45° for 6 hours;
[0042] The next day, water the bedding material of raising cage with water, and the time that mouse is placed in raising cage is 6h;
[0043] On the third day, the animals were placed in the dark box, and the light and dark lights were exchanged every 7 minutes, ...
Embodiment 2
[0051] 1) Observe the signs of the mice with atherosclerotic liver depression syndrome prepared in this application, with normal mice as reference, the observation results are shown in Table 1.
[0052] 2) Increase in body weight and changes in blood lipid metabolism in mice
[0053] According to the digital table method, ApoE- / - mice were randomly divided into normal group, high-fat group and liver depression group, and another 10 inbred C57BL / 6 / J mice of the same week age were set as blank group; blank control group The normal group and the normal group were fed with common feed; the high-fat group was fed with western dietary feed containing 21% fat and 0.15% cholesterol (Co60γ sterilized irradiation treatment, Beijing Keao Xieli Co., Ltd.), without other intervention; Apply for the preparation of atherosclerotic liver depression syndrome mice.
[0054] As shown in Table 2, comparing the body weight of the mice before and after the experiment, it was found that the mice in...
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