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A kind of saponin compound extracted from Fritillaria japonicus and its method and application

A compound, the technology of Fritillaria, applied in the field of phytochemistry, achieves good reference value and rich diversity

Active Publication Date: 2020-05-19
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Because plant-derived compounds have great potential for discovering new drugs, anti-tumor drugs derived from plants have gradually been paid attention to in recent years. There is still a great development prospect and space in the active ingredients of Fritillaria japonicus. For this reason, it is imperative to develop and develop new anti-tumor drug ingredients in Fritillaria japonicus

Method used

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  • A kind of saponin compound extracted from Fritillaria japonicus and its method and application
  • A kind of saponin compound extracted from Fritillaria japonicus and its method and application
  • A kind of saponin compound extracted from Fritillaria japonicus and its method and application

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Embodiment 1

[0042] The present embodiment proposes a saponin compound extracted from Fritillaria japonicus, the molecular formula of the saponin compound is C 30 h 48 o 6 , is a white powder, odorless, the specific structure is as follows:

[0043] .

[0044] The saponin compound is obtained by extracting and separating the dried tubers of Fritillaria japonicus, and the specific extraction steps are as follows:

[0045] A. Using the dried tubers of Fritillaria japonicus as raw materials, the total saponins of Fritillaria japonicus are obtained after successively crushing, extracting with ethanol, concentrating under reduced pressure, extracting, and filtering;

[0046] B. hydrolyzing the total saponins of Fritillaria japonicus in a sulfuric acid water bath, cooling and filtering to obtain a hydrolyzed solid;

[0047] C. Dissolve the hydrolyzed solid in methanol and mix the sample with silica gel. Take the silica gel mixed sample and perform normal phase silica gel column chromato...

Embodiment 2

[0050] The present embodiment proposes a saponin compound extracted from Fritillaria japonicus, the molecular formula of the saponin compound is C 30 h 48 o 6 , is a white powder, odorless, the specific structure is as follows:

[0051] .

[0052] The saponin compound is obtained by extracting and separating the dried tubers of Fritillaria japonicus, and the specific extraction steps are as follows:

[0053] A. Use the dried tubers of Fritillaria japonicus as raw materials, grind them, heat and reflux them with 60% W / W ethanol, which is 6 times its weight, and extract them under reflux for 3 times, then concentrate under reduced pressure to no alcohol, and then disperse the obtained extracts with water Afterwards, use n-butanol to extract 3 times in equal volumes, concentrate the n-butanol phase at 45° to a small volume and then precipitate, and filter to obtain the total saponins of Fritillaria japonicus.

[0054] B. The total saponins of Fritillaria japonicus obtaine...

Embodiment 3

[0061] The present embodiment proposes a saponin compound extracted from Fritillaria japonicus, the molecular formula of the saponin compound is C 30 h 48 o 6 , is a white powder, odorless, the specific structure is as follows:

[0062] .

[0063] The saponin compound is obtained by extracting and separating the dried tubers of Fritillaria japonicus, and the specific extraction steps are as follows:

[0064] A. Use the dried tubers of Fritillaria japonicus as raw materials, grind them, heat and reflux them with 80% W / W ethanol with a concentration 12 times its weight, and extract them under reflux for 5 times, then concentrate them under reduced pressure until they are alcohol-free to obtain extracts, and disperse the obtained extracts with water Afterwards, use n-butanol to extract 4 times at equal volumes, concentrate the n-butanol phase at 45° to a small volume and then precipitate, and filter to obtain the total saponins of Fritillaria japonicus.

[0065] B. The to...

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Abstract

The invention discloses a saponin compound extracted from rhizoma bolbostemmatis and a method and application thereof. The method comprises the steps that dry tuber of rhizoma bolbostemmatis is used as a raw material, after the raw material is subjected to the steps of ethanol extraction, decompression and concentration, extraction, hydrolysis, mixing with silica gel to make a sample, positive phase silica gel column chromatography and the like, a new medicinal compound with pharmacological activity is extracted, the new medicinal compound with pharmacological activity is self-named as virgaureagenin F, can be used for inhibiting the growth of tumor cells, not only provides a treatment scheme for the research of existing tumor cells, but also provides a basis for the development of new drugs derived from rhizoma bolbostemmatis.

Description

technical field [0001] The invention relates to a saponin compound extracted from Fritillaria japonicus and its method and application, in particular to a saponin compound with pharmacological activity extracted and separated from Fritillaria japonicus medicinal material, its method and application, and belongs to the technical field of phytochemistry. Background technique [0002] Bolbostemma paniculatum (Maxim.) Franquet is a plant of the genus Cucurbitaceae Pseudo-Fritillaria, which is a climbing herb and can be found on hillsides or plains. It is a traditional Chinese medicine. There are 2 species and 1 variant of Pseudomonas, which are unique to my country. They are mainly produced in Henan, Hebei, Shanxi, Inner Mongolia, Shandong, Shaanxi, Gansu, Yunnan, Sichuan and other places. They are widely distributed in the wild, and artificial cultivation has been successful. The yield Quite abundant. The tubers are used for medicinal purposes, and the dry tubers are hard and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61P35/00
CPCC07J63/008
Inventor 何文彬白兰辉李波张巨琼王晨曦张佳卉徐仕银李旺刘丁文焕松夏柯钟钰
Owner CHENGDU PUSH BIOLOGICAL TECH
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