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Semen litchi particle HPLC fingerprint establishing method

A technology of fingerprints and establishment methods, which is applied in the field of establishing HPLC fingerprints of litchi core granules, can solve the problems that cannot truly reflect the quality consistency of litchi core granules, and achieve safety and effectiveness, strong operability, good repeatability

Inactive Publication Date: 2018-06-15
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current quality control standards for litchi kernel granules can no longer truly reflect the inherent quality of litchi kernel granules and the consistency of product quality between batches

Method used

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  • Semen litchi particle HPLC fingerprint establishing method
  • Semen litchi particle HPLC fingerprint establishing method
  • Semen litchi particle HPLC fingerprint establishing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Instrument: Agilent high performance liquid chromatography.

[0025] Medicinal materials: lychee kernel granules provided by Guangxi Hepu Guoxiangyuan Food Co., Ltd.; control medicinal material lychee kernel was purchased from Guangxi Yulin Xinkang Medicinal Materials Co., Ltd.

[0026] Reagents: mobile phase A methanol is chromatographically pure, water is ultrapure water, and other reagents are analytically pure.

[0027] Preparation of the test solution: Weigh an appropriate amount of lychee core particles, grind them finely, accurately weigh about 1.0 g, add water to dissolve, heat in a boiling water bath for 20 minutes, cool and centrifuge, take the supernatant and filter it with a microporous membrane to prepare Obtain the test solution.

[0028] Preparation of the reference solution: Accurately weigh 1.0 g of the litchi core reference medicinal material, add water to dissolve, heat in a boiling water bath for 20 minutes, cool, centrifuge, take the supernatant an...

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Abstract

The invention provides a semen litchi particle HPLC fingerprint establishing method which comprises the steps: preparing a test sample, determining a chromatographic condition and utilizing a high performance liquid chromatograph to measure to obtain a semen litchi particle HPLC fingerprint. Chemical information of the semen litchi particles can be comprehensively and accurately expressed. The fingerprint has the characteristics that a separation degree and a peak pattern of a common peak are better, repeatability is good, operability is strong, and quality of the semen litchi particles can beeffectively controlled.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicine preparations, in particular to a method for establishing HPLC fingerprints of litchi kernel granules. Background technique [0002] Litchi seed is the dry mature seed of Sapindaceae plant Litchi. It is sweet in taste, slightly bitter, and warm in nature. Return liver, kidney meridian, have the function of promoting qi circulation and dispelling stagnation, dispelling cold and relieving pain, used for colic due to cold, abdominal pain, testicular swelling and pain. According to research, the main chemical components of litchi kernels are litchi kernel saponins, flavonoids, volatile oils, organic acids and esters, amino acids, sugars and trace elements. [0003] Although the development of traditional Chinese medicine is developing rapidly, the active ingredients of most traditional Chinese medicines are still unclear. The current quality control standards for litchi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赵立春
Owner GUANGXI UNIV OF CHINESE MEDICINE
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