Pharmaceutical application of collagen peptide, cucurbitacin oral micelles and preparation method thereof
A technology of collagen peptide and cucurbitacin, which is applied in the field of medicine, can solve the problems of complicated preparation methods and high cost, and achieve the effect of improving bioavailability, low cost and good inhibition rate
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Embodiment 1
[0046] Preparation of positively charged collagen peptides: Weigh 5 g of D152 cation exchange resin and place it in a conical flask, add 10 mL of collagen peptide aqueous solution with a mass concentration of 0.1 g / mL, wherein the molecular weight of the collagen peptide is 3000 Da, After oscillating and adsorbing in a shaker at 40°C for 3 hours, collect the adsorbed resin, elute with 10 mL of 0.2 mol / L sodium chloride aqueous solution for 2 hours, filter, and discard the eluent; mol / L sodium chloride aqueous solution repeats the same steps for elution 3 times, collects the eluate, and combines the obtained eluents to obtain the total solution, concentrates to remove salt, dialyzes with a dialysis bag, and freeze-dries to obtain a positively charged collagen peptide , its appearance is white soft powder with obvious crystal luster.
Embodiment 2
[0048] Analysis of the amino acid composition of collagen peptides: Weigh 0.1 g of commercially available collagen peptides and the collagen peptides prepared in Example 1, add 10 ml of HCl with a concentration of 6 mol / L, oscillate and mix well, seal and place in an oven at 110°C Hydrolyze for 24 hours, filter the hydrolyzate, take 500μl filtrate, vacuum dry at 55°C, add 0.02mol / L HCl to the dried sample to dissolve and dilute, mix the standard solution with amino acid for ninhydrin colorimetry, determine the degree of sample dilution, use 0.22 μm microporous membrane filtration, automatic amino acid analyzer for analysis, the composition and content changes of different amino acid types in collagen peptides before and after separation are shown in Table 1;
[0049] Table 1 Amino acid composition analysis of 3000Da commercially available collagen peptides and positively charged collagen peptides
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[0051]
[0052] The amount of acidic amino acid and basic amino ...
Embodiment 3
[0054] Preparation of collagen peptide-total cucurbitacin mixed nanomicelles:
[0055] 1) Prepare 5 mg / mL total cucurbitacin methanol solution (based on the concentration of cucurbitacin B, the content of cucurbitacin B is 65%), take 2 mL of the above solution, add 48 mg lecithin, and stir at 40 ° C for 45 min;
[0056] 2) Weigh 71 mg of caprylic capric acid macrogol glyceride and 29 mg of polyethylene glycol 15-hydroxystearate, add them to the mixed solution obtained in step 1), and continue stirring for 45 min at the same temperature;
[0057] 3) Evaporate the solution obtained in step 2) to remove methanol, blow with nitrogen to completely remove the organic solvent, and obtain a uniformly distributed transparent drug-containing lipid film;
[0058] 4) Add 17 mL of purified water to dissolve the lipid film obtained in step 3), and after ultrasonic hydration at 40°C for 1 hour, centrifuge at 10,000 rpm for 20 minutes;
[0059] 5) Take the supernatant obtained in step 4), ad...
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