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A rna interference method of the book lice sod1 gene

A technology of RNA interference and book lice, applied in DNA/RNA fragments, recombinant DNA technology, botanical equipment and methods, etc., can solve the problems of difficult application of RNA interference technology system

Inactive Publication Date: 2021-08-17
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, the RNA interference technology system successfully applied to other insects is difficult to apply to tiny storage booklice such as bookhopper

Method used

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  • A rna interference method of the book lice sod1 gene
  • A rna interference method of the book lice sod1 gene
  • A rna interference method of the book lice sod1 gene

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Starvation treatment of booklice adults

[0039] Use a No. 0 writing brush to gently pick healthy, uniformly developed adults of Booklice nymphophilus into plastic culture boxes with a diameter of 2.5 cm and a height of 1 cm, with 150 heads per box, 16 boxes in total. The artificial climate incubator is controlled in complete darkness, the temperature is set at 27.5°C, the relative humidity is at 75%-80%, and the starvation treatment is performed for 24 hours to increase the amount of dsRNA eaten by the book lice.

[0040] (2) Feed preparation

[0041] Select clean and plump common wheat, dry it at 60°C for 12 hours to achieve the effect of killing mites and sterilization, and grind it to make whole wheat flour; for yeast powder, you can buy commercially available yeast flakes and grind it into fine powder; the whole wheat flour: Yeast powder: Skimmed milk powder is mixed in a ratio of 10:1:1 and stirred evenly.

[0042] (3) Preparation of dsRNA dry powder

[004...

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Abstract

The invention relates to the fields of plant protection and biotechnology, and discloses an RNA interference method for the SOD1 gene of the book lice. Based on the SOD1 gene of the book lice, a specific dsRNA is synthesized, and the liquid dsRNA is made into a dry powder by using a freeze dryer. The book lice feed was mixed at a ratio of 1:10; the SOD1 gene of Book lice was silenced by the feeding method, which reduced the ability of Book lice to resist adversity, which could assist in realizing the ecological regulation of Book lice to a certain extent. The invention has a novel and reasonable design, and solves the difficult problem of book lice RNA interference technology. The invention can silence the SOD1 gene of the book lice, and the silencing efficiency can be as high as 95% within 48 hours. The capture of the RNA interference technology of the book lice provides a new idea for the formulation of the ecological regulation technology of the book lice and the research of functional genes in the future.

Description

technical field [0001] The invention relates to the fields of plant protection and biotechnology, in particular to an RNA interference method for the SOD1 gene of the book lice. Background technique [0002] The booklice (Liposcelis bostrychophila) belongs to the order Psocoptera, the family Liposcelididae, and the genus Liposcelis. It is a stored product pest that occurs widely and causes serious losses worldwide. Booklice not only directly feed on stored materials, but also can carry and spread germs and cause allergic reactions, directly endangering people's health. Due to its wide range of feeding, strong activity, high reproductive rate and parthenogenesis, long-term unreasonable use of chemical agents in the prevention and control has resulted in a high risk of a variety of stored grain protective agents and fumigants. Tolerance or drug resistance is very difficult to prevent and control. Superoxide dismutase (SOD) is an enzyme that can catalyze the conversion of sup...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/113A01K67/033A23K20/153A23K50/90A01G13/00
CPCA01G13/00A01K67/0335A01K2207/05A01K2207/25A01K2227/706A01K2267/0393A23K20/153A23K50/90
Inventor 魏丹丹郎宁苗泽青肖林帆王进军
Owner SOUTHWEST UNIV
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