Primer set, site and method for sex identification of soft-shelled soft-shelled turtle

Abstract

The invention discloses pelodiscus sinensis sex identification specific primers and a method. The two pairs of primers in a sex identification primer system are a pair of female specific primers and apair of positive reference primers which can be amplified by using male and female genomic DNA. Among the primers, the female specific primers can only stably amplify a 196 bp band in the female genomic DNA, and the band cannot be amplified in the male genomic DNA; the positive reference primers can amplify a 482 bp band in the male and female genomic DNA. Thus, in a PCR reaction system, the female genomic DNA can amplify two bands of 196 bp and 482 bp, while the male can only amplify a band of 482 bp. The use of the primers facilitates the correct identification of the sex of pelodiscus sinensis, especially the sex identification of the pelodiscus sinensis in the embryonic stage which is difficult to distinguish through naked eyes. The primers lay the foundation for the study of the pelodiscus sinensis, and also provide an important tool for the breeding and artificial breeding of the pelodiscus sinensis.

Description

technical field [0001] The invention relates to a specific primer for amplifying the specific sequence of the Chinese soft-shelled turtle W chromosome-linked gene SBNO1 and a positive reference primer capable of amplifying a 482bp sequence from both male and female Chinese soft-shelled turtle genome DNA, in particular to a primer set and identification for sex identification of Chinese soft-shelled turtle method. Background technique [0002] Chinese soft-shelled soft-shelled turtle (Pelodiscus sinensis) belongs to the genus Trionychidae of the order Testudinata of the class Reptilia, and is also known as soft-shelled turtle, turtle, water fish, etc. It is an important aquatic reptile. It is listed as a Vulnerable (VU) species on the IUCN Red List of Threatened Species. It is widely distributed in other provinces in my country except Xinjiang, Tibet and Qinghai, and has also been introduced in Vietnam, Japan, Korea, Timor Island and the Hawaiian Islands. [0003] Chinese s...

AI Technical Summary

Problems solved by technology

At present, there is no effective molecular method to identify the sex of soft-shelled soft-shelled turtles. However, it is difficult and time-consuming to identify the sex of soft-shelled soft-shelled turtle embryos and juvenile soft-shelled turtles by other technical means, such as karyotype analysis or comparative genomic hybridization (CGH) analysis. certain error rate

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