126 results about "Southeast Asian box turtle" patented technology

An indoor-outdoor multi-crops polyculture method for shrimp, soft-shelled turtle and fish includes building an upper greenhouse culture pond and a lower outdoor intensive culture pond, and using a transfer pipe to communicate the upper greenhouse culture pond and the lower outdoor intensive culture pond; throwing desalted giant tiger prawn in the upper greenhouse culture pond in mid-March, and keeping water temperature at 25 DEG C; pouring water into the lower outdoor intensive culture pond prior to throwing in silver carp, bighead carp and Chinese soft-shelled turtle for breeding in early May; after seedlings grow to about 5cm 50-60 days after culture of the giant tiger prawn in the upper greenhouse culture pond, transferring young shrimps in the upper greenhouse culture pond into the lower outdoor intensive culture pond for breeding through the transfer pipe, harvesting first crop from late June to early July; and harvesting the silver carp, the bighead carp and the Chinese soft-shelled turtle in November to December after continuous three-crop culture of the giant tiger prawn. The indoor-outdoor multi-crops polyculture method for shrimp, soft-shelled turtle and fish breaks through the bottleneck of existing culture production and benefit.

The invention discloses an ecological cycle production system of turtles, fishes, rice and vegetables. The ecological cycle production system is characterized in that a planting and breeding combined three-stage circulation system is formed by interbreeding edible fish and interplanting rice and aquatic vegetables in an environment mainly used for breeding Chinese soft shell turtles. The manner of planting and breeding combination, mutualism and ecological prevention and control can be used for better solving the problems of more serious endogenous pollution and the like pervasively existing in the current turtle (fish) breeding water body as well as pervasive problems of low quality and efficiency for rice planting and the like, thereby greatly reducing the utilization ratio of pesticides in the environment and realizing the comprehensive production target of ecological cycle agriculture with the advantages of saving resources and being environmentally-friendly, high in quality of products, economical and efficient.

The invention relates to a PCR amplification primer and a method for rapidly identifying a genetic sex of Chinese softshell turtle. A sequence of the primer is as shown by SEQ ID No.1 and SEQ ID No. 2. The identification method adopts Chinese softshell turtle DNA to be identified as a template and utilizes the primer to perform PCR amplification, the electrophoresis detection is performed for an amplification product, when the amplification product has a strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the female (i.e. the type of sex chromosome is ZZ);and when the amplification product has the strip of 1533bp and the strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the male (i.e. the type of the sex chromosome is ZW). The invention also provides a kit comprising the primer. The PCR amplification primer, the method and the kit are simple, rapid, low in cost, and capable of providing reference evidence for developing a Chinese softshell turtle genetic sex marker, identifying the genetic sex, studying the sex decision mechanism and studying the artificially-controlled Chinese softshell turtle sex differentiation.

The invention discloses a method for integrated culture of rice-finless eel-crayfishp-trionyx sinensis. The method comprises the following steps: placing myriophyllum aquaticum in a ring-shaped ditchof a paddy field; putting crayfish seedlings in April or August, putting trionyx sinensis seedlings and cipangopaludina cahayensis in May, and putting finless eel seedlings in late June to early July;performing feeding; and catching crayfish in early June or early October, and catching finless eels and trionyx sinensis in November to December. According to the method for integrated culture of therice-finless eel-crayfish-trionyx sinensis, ecosystem biodiversity of the paddy field is improved, cannibalism degree of the crayfish is reduced, requirement for live baits of the finless eels and the trionyx sinensis is satisfied; and the method has the advantages of higher biological diversity, easy catching, adequate supply of live baits, more stable culture yields, higher economic benefits, and the likes.

The invention discloses a PCR (Polymerase Chain Reaction) amplification primer for quickly distinguishing the genetic sex of a Chinese softshell turtle. The PCR amplification primer comprises a pair of sex specificity molecular markers and a pair of reference gene primers. The invention further discloses a PCR method for quickly distinguishing the genetic sex of the Chinese softshell turtle and akit for quickly distinguishing the genetic sex of the Chinese softshell turtle. The amplification primer can distinguish the genetic sex of the Chinese softshell turtle in the early stage. The methodis low in cost and easy and convenient to operate and can quickly distinguish a large batch of turtles, and a result is intuitive and accurate.

The present invention discloses a whole-course pollution-free and ecological cultivation method for Chinese softshell turtles. The cultivation method comprises the following steps of: temporarily cultivating juvenile turtles; building a intensive cultivating tank for the juvenile turtles in an pond outside; stocking the juvenile turtles; respectively feeding the juvenile turtles; water quality management; overwintering management; and disease prevention. According to the whole-course pollution-free and ecological cultivation method for Chinese softshell turtles disclosed in the present invention, hatching juvenile turtles; after temporarily cultivation of the juvenile turtles, stocking the juvenile turtles into an outside pool simulating living environment of turtles in the nature; cultivating turtles in different stages in the pool outside for three years or more with scientific released density; in the whole cultivation process, water quality remaining good without any pollution and uses of forbidden drugs, as well feed that is balanced in nutrition proportion without any forbidden additives is used, with fresh and live baits as auxiliary feed; accordingly, pollution-free turtles with good quality and commercial specification being over 1.5 kg each turtle are cultivated; and the cultivated turtles are high in nutrition and medical value.

The invention discloses an environment-friendly pond culture method for Chinese soft-shell turtles. The method comprises the steps as follows: (1), pond selection; (2), pond preparation and aquatic plant configuration; (3), stocking density; (4), feed and feeding; (5), water quality regulation and daily management; and (6), adult turtle catching. When the environment-friendly pond culture method is applied to culture of the Chinese soft-shell turtle, not only can better economic benefit be achieved, more importantly, the pond water quality is maintained better all along with the culture mode, almost no culture waste water is drained, and the ecological benefit is quite obvious. Under the condition that other culture varieties suffer from frequent diseases and the culture risk is higher at present, the environment-friendly pond culture method for the Chinese soft-shell turtles is a good way for fishermen to become rich.

The present invention discloses a method for culturing the young Chinese soft-shelled turtles. Said method includes the following steps: for newly incubated young soft-shelled turtles using living water fleas and earthworm to feed them in indoor clear water temporary nursery; and for small young soft-shelled turtles using artificial feed whose coarse protein content is above 3-5% to feed them, firstly feeding them in greenhouse, then culturing them in outdoor pond.

The invention claims a greenhouse cultivation technology of soft-shelled turtles, belonging to the field of a cultivation technology. The greenhouse cultivation technology is characterized by carrying out cultivation according to the following steps: (1), incubating soft-shelled turtle eggs, incubating the soft-shelled turtle eggs on a magnet-combined sponge for 45 days during the period from August to October; (2), raising seedlings, placing newly incubated seedlings in a greenhouse soft-shelled turtle tank, breeding till April in the next year, and transferring to a transitional greenhouse soft-shelled turtle tank to feed during the period from April to May; (3) in May, choosing large soft-shelled turtles with good body color as parent soft-shelled turtles when the temperature inside the greenhouse is basically equal to the temperature outside, placing the parent soft-shelled turtles into a standby parent soft-shelled turtle tank to feed for one year, and placing the rest of soft-shelled turtles in the external soft-shell turtle tank to feed till October, and then capturing the soft-shelled turtles to sell in the market. According to the greenhouse cultivation technology of the soft-shelled turtles, the domestication of the soft-shelled turtles cultivation can be realized, the parent soft-shelled turtles and the soft-shell turtle eggs are homegrown, therefore, the cultivation is convenient, the cost is saved, large-scale cultivation is facilitated to realize, and the economic benefit is increased.

The invention relates to a probiotic feed additive for Chinese softshell turtles and a preparation method of the probiotic feed additive. The probiotic feed additive is prepared from probiotics and carrier fillers, wherein the carrier fillers are sterilized wheat bran, and the probiotics include bacillus subtilis, bacillus natto, Lactobacillus casei and candida utilis with the ratio of (0.4-0.6):(0.4-0.6):(0.8-1.2):(0.8-1.2), and the probiotics are added in the aseptic carrier fillers to prepare probiotic powder. The probiotic feed additive can improve the growth performance of the Chinese softshell turtles, reduce the food coefficient, improve the immunological function and reduce the diseases, has the advantages of low cost, no pollution, no drug resistance, no residue and the like, and is environmentally friendly.

The invention discloses pelodiscus sinensis sex identification specific primers and a method. The two pairs of primers in a sex identification primer system are a pair of female specific primers and apair of positive reference primers which can be amplified by using male and female genomic DNA. Among the primers, the female specific primers can only stably amplify a 196 bp band in the female genomic DNA, and the band cannot be amplified in the male genomic DNA; the positive reference primers can amplify a 482 bp band in the male and female genomic DNA. Thus, in a PCR reaction system, the female genomic DNA can amplify two bands of 196 bp and 482 bp, while the male can only amplify a band of 482 bp. The use of the primers facilitates the correct identification of the sex of pelodiscus sinensis, especially the sex identification of the pelodiscus sinensis in the embryonic stage which is difficult to distinguish through naked eyes. The primers lay the foundation for the study of the pelodiscus sinensis, and also provide an important tool for the breeding and artificial breeding of the pelodiscus sinensis.

The invention discloses a cultivation method of a cuora flavomarginata. The cultivation method of the cuora flavomarginata comprises four stages, namely, (1) an incubation period, (2) an infant cuora flavomarginata cultivation period, (3) an infant cuora flavomarginata nurture period, and (4) a grown cuora flavomarginata nurture period. The method is characterized in that the survival rate of the cuora flavomarginata can be increased to 90 percent by adopting the novel cultivation method of the cuora flavomarginata in the three periods (1) the incubation period, (2) the infant cuora flavomarginata cultivation period, (3) the infant cuora flavomarginata nurture period in virtue of precise and reasonable arrangement of a cultivation device of the cuora flavomarginata and scientific management, and the death rate of the cuora flavomarginata during the infant cuora flavomarginata period and a young cuora flavomarginata period is reduced greatly; and besides, not only is the cultivation cost low, the management is scientific, the quality of a grown cuora flavomarginata, the ornamental value and the medicinal value are high, but also human constant supervision is not required, the labor force is reduced, the adjustment of the temperature and humidity is convenient, timely and reliable, and large scale cultivation is suitable.

The invention discloses a cultivation method for cultivating Chinese softshell turtles and yellow-head catfish meats. The method comprises the following steps: (1) draining water in a tank and exposing to sun, sterilizing by uniformly scattering calcined lime for sterilization, applying fermented organic manure after 2-3 days, and equipping an aerator; (2) breeding yellow-head catfish meat fingerlings which are 2-3cm per fish, and Chinese softshell turtle fingerlings which are 0.25-0.5kg per fish, regulating the amount of fodders once every 7-10h, and adding vitamins and/or saccharomycetes which take up to 5-10% of fodders in the fodders every 10-15 days; (4) after 7 days of breeding the fingerlings, spraying trichodina once, filling fresh water every 7-10 day once, and keeping the transparency of the cultivation water to be greater than 30cm; and (5) fishing once every 1-3 months. The healthy cultivation method for cultivating Chinese softshell turtles and yellow-head catfish meats with high efficiency is used for cultivating, so as to reasonably determine the breeding specification and cultivation density, improve the utilization factors of the tank water, control the cultivation risk, improve the comparative benefit of pond culture, therefore, the biological healthful aquaculture of cultivating Chinese softshell turtles and yellow-head catfish meats can be realized.

The invention discloses a method for managing pond aquaculture of Chinese softshell turtles, and relates to the technical field of transformations of aquaculture technologies. The method is characterized by comprising the following steps: selecting pond conditions, making a back drying table, making a feed table, stocking young Chinese softshell turtles, throwing feed for breeding, and managing the water quality. The method is reasonable, convenient to operate and clear in proportion.

The invention discloses a breeding method for Chinese tortoise, comprising: 1) Turtle pond construction: the turtle pond includes a heat preservation pool and a culture pond, and the culture pond is fixedly embedded in the heat preservation pond through a rectangular opening; the culture pond is provided with a second A water inlet pipe and a first water outlet, the first water inlet pipe is connected to the first constant temperature water tank with 24-26°C water, the first water outlet is connected to the heat preservation pool, and the heat preservation pool is provided with a second water inlet pipe and a second outlet The water inlet, the second water inlet pipe is connected to the second constant temperature water tank with 27-29°C water, and the second water outlet is connected to the outside of the heat preservation pool; 2), preparation before entering the pool: put the quicklime into the breeding pool and add water, after exporting , feed 27~29 ℃ of water in the insulation pond, feed 24~26 ℃ of water in the breeding pond; 3), Chinese tortoise breeding. The present invention maintains the stability of the entire environment temperature through the structure in which the entire culture pool is fixedly embedded in the heat preservation pool, improves the survival rate of the Chinese tortoise, and contributes to the growth of the Chinese tortoise.

The present invention discloses a temperature and humidity control method of trionyx sinensis incubation room. The temperature and humidity control method comprises the following control steps: (1) providing a heating apparatus, a humidifying apparatus and a ventilation apparatus in the incubation room, and providing a water sprayiing apparatus at the roof of the incubation room; and (2) by virtue of a signal collected by the temperature sensor and humidity sensor provided in the incubation room, controlling the temperature of the incubation room at 28-30 DEG C and the humidity at 78%-85% by controlling the heating apparatus, the humidifying apparatus, the ventilation apparatus and the water spraying apparatus. According to the temperature and humidity control method of trionyx sinensis incubation room disclosed by the invention, after trionyx sinensis eggs in a trionyx sinensis pond are collected in a unified mode, the method is utilized to perform incubation; and the method has characteristics of a high incubation rate and short incubation time.

The invention discloses a pelodiscus sinensis fodder containing low-protein fish meal added with methionine hydroxy analog-Ca. The raw material of the pelodiscus sinensis fodder comprises white fish meal, soy protein concentrate, fermented soybean meal, extruded soybean, chicken powder, squid powder, beer yeast, alpha-starch, gluten flour, zeolite powder, multivitamin, a compound mineral substance, choline chloride, glycine betaine, sodium dihydrogen phosphate and methionine hydroxy analog-Ca. According to the pelodiscus sinensis fodder disclosed by the invention, the methionine hydroxy analog-Ca is taken as the additive and is added into the pelodiscus sinensis fodder containing low-protein fish meal, so that the amino acid balance in the fodder is effectively kept, the growth promotion effect is excellent and the nutritional requirement for the growth of the pelodiscus sinensis is met. Compared with the traditional pelodiscus sinensis fodder taking fish meal as the main protein source, the pelodiscus sinensis fodder containing low-protein fish meal added with methionine hydroxy analog-Ca has the advantages that the protein content of the fodder is less than or equal to 44% and the additive amount of the fish meal is less than or equal to 26%. The fodder provided by the invention is capable of saving the dosage of the fish meal in the pelodiscus sinensis fodder and lowering the cost of the fodder, is ecological and environmentally friendly, and is capable of increasing the comprehensive breeding benefit.

The invention discloses a hatching device and method for Chinese softshell turtle eggs. The hatching device comprises a hatching house controller and a water tank, and a heater is arranged in the water tank; more uniform heating or cooling is achieved through floor heating and hot air or cold air blowing in an air duct of the side wall of a hatching house; the temperature and the humidity in the hatching house are changed and adjusted by detecting the temperature and the humidity in the hatching house in real time and comparing detected temperature values and detected humidity values with a preset temperature value and a preset humidity value, therefore, it is guaranteed that the temperature and the humidity in the hatching house are within the preset ranges, softshell turtle egg hatching and control over the male and female of hatched Chinese softshell turtles are guaranteed, the hatching rate is increased, and the hatching time is shortened.

The invention discloses a feed additive for trionyx sinensis and a preparation method of the feed additive. For specific conditions of the trionyx sinensis, the feed additive is prepared through combination of modern bioprocesses according to a certain rule and on the basis of the theory of traditional Chinese herbal medicines, is nature in sources, can effectively increase the nutritional value of a basic feed, improve immunity of the trionyx sinensis, guarantee health of the trionyx sinensis, remarkably improve the flavor and nutritional value of the trionyx sinensis, take effects quickly, and greatly improve the quality of the trionyx sinensis within two months. In addition, the living bacterium additive is long in guarantee period and can be stored for 18 months at normal temperature with, and the living bacterium survival rate can still reach 90% or above.