Method for producing early gonad paraffin section of Chinese soft shell turtle

A technology of paraffin section and preparation method, which is applied in the preparation of test samples and other directions, can solve the problems of easy loss, light color of gonad tissue, and difficult to observe, and achieves low cost, wide source, and avoids difficult operation and observation. Effect

Active Publication Date: 2014-07-30
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the small size of the gonads in the early stage of soft-shelled turtles, the following problems usually occur during the traditional paraffin sectioning operation: (1) the gonad tissue is light in color, and it is difficult to be observed after the tissue is transparent, and it is easy to lose; (2) the operation It is easy to damage the gonad during the process, thereby destroying the integrity of the gonad; (3) The gonad tissue is small in size and not easy to operate

Method used

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  • Method for producing early gonad paraffin section of Chinese soft shell turtle
  • Method for producing early gonad paraffin section of Chinese soft shell turtle
  • Method for producing early gonad paraffin section of Chinese soft shell turtle

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1. Pretreatment and fixation of soybean products

[0035] Soy products use tofu with high hardness. Large pieces of tofu are first cut into square plates with a thickness of 1cm, and then cut into a cross-sectional area of ​​about 1cm. 2 thin strips, lightly cross-stacked in a resealable plastic box. Fix with 4% paraformaldehyde fixative for 6h, then soak in 1% hydrochloric acid solution for 0.5h, rinse in running water and soak in 70% alcohol solution.

[0036] 2. Forming and punching

[0037] Cut the pretreated strips of soy products into appropriate thickness, and use hard capillary tubes to punch holes. The size of the holes can be controlled by capillary tubes or tubes of different diameters and shapes. The tube wall should not pursue smooth cutting. The irregular shape is conducive to the fixation of the gonads in the later stage.

[0038] 3. Isolation and pre-embedding of the embryonic gonads of Chinese soft-shelled turtle

[0039]Take the soft-shelled turtle...

Embodiment 2

[0047] 1. Pretreatment and fixation of soybean products

[0048] Soy products use vegetarian chicken with high hardness. Large pieces of vegetarian chicken are first cut into square plates with a thickness of 2cm, and then cut into a cross-sectional area of ​​about 2cm 2 thin strips, lightly cross-stacked in a resealable plastic box. Fix with formalin solution for 24h, then soak in 7% hydrochloric acid solution for 5h. Rinse in running water and soak in 80% alcohol solution.

[0049] 2. Forming and punching

[0050] Cut the pretreated strips of soy products into appropriate thickness, and use hard capillary tubes to punch holes. The size of the holes can be controlled by capillary tubes or tubes of different diameters and shapes. The tube wall should not pursue smooth cutting. The irregular shape is conducive to the fixation of the gonads in the later stage.

[0051] 3. Isolation and pre-embedding of the embryonic gonads of Chinese soft-shelled turtle

[0052] Take the so...

Embodiment 3

[0060] In the steps of pretreatment and fixation of soybean products, Born's solution was used for fixation, and the rest of the steps were the same as in Example 1.

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Abstract

The invention relates to a method for producing an early gonad paraffin section of Chinese soft shell turtle, which comprises the following steps: 1)fixing a sample, 2)dehydrating and transparentizing, 3)performing paraffin infiltration and embedding, 4)slicing and spreading the section, and 5)dyeing and sealing the section. The method is characterized in that the sample in the step 1) is embedded in a bean product pretreated by a stationary liquid. Compared with prior art, the method has the advantages that the bean product can be taken as a pre-embedding material for embryo stage gonad paraffin section of Chinese soft shell turtle, the source is wide, cost is low, through the pre-embedding, the embryo stage gonad can be better embedded in bean product, and subsequent tissue embedding and slicing are not influenced. The method avoids the disadvantages in a traditional method, and overcomes the disadvantages of difficult operation and observation of early gonad paraffin section of Chinese soft shell turtle; in addition, the pre-embedding gonad position is fixed and several gonad tissue can be processed at a time, so that large scale slicing and analysis are convenient to operate, and the method is especially suitable for researches by aiming at different individuals.

Description

technical field [0001] The invention relates to a method for making paraffin slices, in particular to a method for making paraffin slices of early gonads of soft-shelled soft-shelled turtles. Background technique [0002] The primordial germ cells of reptiles differentiate and migrate into the genital ridge or differentiate into germ cells in the primordial gonad, and the origin of the primordial germ cells can be traced back to the early stage of embryonic development. The migration methods of primordial germ cells into the genital ridge of different animals are different. When the genital ridge (gonad primordium) occurs, the primordial germ cells differentiate in the genital ridge, and the primordial germ cells continue to divide and proliferate during the migration process. With the growth of gestational age, sex differentiation occurs, and the genital ridge differentiates into male testis or female Ovaries, where primordial germ cells form sperm or eggs. [0003] Chines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/36
Inventor 李彩燕钱国英王伟赵彩胜葛楚天尹尚军朴龙斗
Owner ZHEJIANG WANLI UNIV
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