PCR amplification primer, method and kit for rapidly identifying genetic sex of Chinese softshell turtle

Abstract

The invention relates to a PCR amplification primer and a method for rapidly identifying a genetic sex of Chinese softshell turtle. A sequence of the primer is as shown by SEQ ID No.1 and SEQ ID No. 2. The identification method adopts Chinese softshell turtle DNA to be identified as a template and utilizes the primer to perform PCR amplification, the electrophoresis detection is performed for an amplification product, when the amplification product has a strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the female (i.e. the type of sex chromosome is ZZ);and when the amplification product has the strip of 1533bp and the strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the male (i.e. the type of the sex chromosome is ZW). The invention also provides a kit comprising the primer. The PCR amplification primer, the method and the kit are simple, rapid, low in cost, and capable of providing reference evidence for developing a Chinese softshell turtle genetic sex marker, identifying the genetic sex, studying the sex decision mechanism and studying the artificially-controlled Chinese softshell turtle sex differentiation.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, relates to the field of genetic sex (sex chromosome type ZZ / ZW) identification of Chinese soft-shelled turtle, in particular to a PCR amplification primer, an identification method and a kit capable of quickly identifying the genetic sex of Chinese soft-shelled turtle. Background technique [0002] Chinese soft-shelled turtle (Trionyx sinensis) is a rare aquatic product in my country. Its meat is delicious, nutritious and nourishing. It is an important freshwater farmed animal in my country, and its output ranks first in the world. my country's soft-shelled turtle commercial breeding began in the late 1970s, the 1980s was the accumulation stage, and the 1990s entered a stable development period. At present, the Chinese soft-shelled turtle aquaculture industry has formed a large-scale and influential special aquaculture industry, and the market price of its wild soft-shelled turtle is sti...

AI Technical Summary

Problems solved by technology

These two technologies are complicated to operate, require high technical level, long identification work time, and high identification cost.

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