PCR amplification primer, method and kit for rapidly identifying genetic sex of Chinese softshell turtle

A Chinese soft-shelled turtle and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of long identification work time, high technical level requirements, and high identification cost, and achieve promotion The effect of application, low cost and simple operation on gender regulation and differentiation of Chinese soft-shelled turtle

Inactive Publication Date: 2018-05-18
HUNAN BIOLOGICAL & ELECTROMECHANICAL POLYTECHNIC +1
View PDF3 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two technologies are complicated to operate, require high techni

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PCR amplification primer, method and kit for rapidly identifying genetic sex of Chinese softshell turtle
  • PCR amplification primer, method and kit for rapidly identifying genetic sex of Chinese softshell turtle
  • PCR amplification primer, method and kit for rapidly identifying genetic sex of Chinese softshell turtle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] DNA samples were taken from skirt tissues of three sexually mature Taiwan soft-shelled soft-shelled soft-shelled soft-shelled turtles, three sexually mature Japanese soft-shelled soft-shelled turtles each, three sexually mature yellow sand soft-shelled soft-shelled soft-shelled turtles each, and three sexually mature Dongting soft-shelled soft-shelled turtles. Genomic DNA extraction kit from animal tissue (Beijing Quanshi Jin Genomic DNA Extraction Kit EE101) was used to extract DNA from the skirt tissue of soft-shelled turtle. The primers designed above were used for PCR amplification of the extracted skirt tissue DNA of soft-shelled turtle, and the amplified products were detected by agarose gel electrophoresis (see Figure 4 ), the results showed that the amplified DNA sample was detected by electrophoresis, and there was a band of 973bp in size, and the genetic sex identification was female (that is, the sex chromosome type was ZZ); there were bands of 973bp and 1533...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a PCR amplification primer and a method for rapidly identifying a genetic sex of Chinese softshell turtle. A sequence of the primer is as shown by SEQ ID No.1 and SEQ ID No. 2. The identification method adopts Chinese softshell turtle DNA to be identified as a template and utilizes the primer to perform PCR amplification, the electrophoresis detection is performed for an amplification product, when the amplification product has a strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the female (i.e. the type of sex chromosome is ZZ);and when the amplification product has the strip of 1533bp and the strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the male (i.e. the type of the sex chromosome is ZW). The invention also provides a kit comprising the primer. The PCR amplification primer, the method and the kit are simple, rapid, low in cost, and capable of providing reference evidence for developing a Chinese softshell turtle genetic sex marker, identifying the genetic sex, studying the sex decision mechanism and studying the artificially-controlled Chinese softshell turtle sex differentiation.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, relates to the field of genetic sex (sex chromosome type ZZ / ZW) identification of Chinese soft-shelled turtle, in particular to a PCR amplification primer, an identification method and a kit capable of quickly identifying the genetic sex of Chinese soft-shelled turtle. Background technique [0002] Chinese soft-shelled turtle (Trionyx sinensis) is a rare aquatic product in my country. Its meat is delicious, nutritious and nourishing. It is an important freshwater farmed animal in my country, and its output ranks first in the world. my country's soft-shelled turtle commercial breeding began in the late 1970s, the 1980s was the accumulation stage, and the 1990s entered a stable development period. At present, the Chinese soft-shelled turtle aquaculture industry has formed a large-scale and influential special aquaculture industry, and the market price of its wild soft-shelled turtle is sti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/686
CPCC12Q1/686C12Q1/6888
Inventor 熊钢王晓清周先文邓灶福康骊曾丹王佩
Owner HUNAN BIOLOGICAL & ELECTROMECHANICAL POLYTECHNIC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap