Probiotic feed additive for Chinese softshell turtles and preparation method thereof

A technology of feed additives and probiotics, applied in biochemical equipment and methods, methods based on microorganisms, animal feed, etc., can solve problems such as fish immunosuppressive drug resistance, poor environmental and human health, and reduced quality of aquatic products , to achieve the effect of enhancing the immune function of the body, improving disease prevention ability, and enhancing absorption and utilization

Inactive Publication Date: 2014-05-14
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use or excessive use will cause side effects such as immunosuppression, drug residues, and drug resistance of pathogenic bacteria in fish, resulting in a decrease in the quality of aquatic products, posing a threat to food safety, and causing adverse effects on the environment and human health.

Method used

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  • Probiotic feed additive for Chinese softshell turtles and preparation method thereof
  • Probiotic feed additive for Chinese softshell turtles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Chinese soft-shelled turtle compound probiotic feed additive preparation

[0042] The compound probiotic feed additive comprises probiotics and adsorption carrier fillers, the probiotics include Bacillus subtilis, Bacillus natto, Candida utilis and Lactobacillus casei, and the adsorption carrier filler is sterilized wheat bran. Its mass ratio in the compound probiotic feed additive (the ratio of each bacterial powder in each gram of additive) is 0.5:0.5:1:1. The amount of compound probiotic preparation powder added in the basic daily dose is added according to the standard of 1000mg-2000mg / (kg basic diet).

[0043] The preparation method of above-mentioned compound probiotic feed additive is:

[0044] The first step: fermentation and cultivation of probiotics.

[0045] (1) Preparation of Bacillus subtilis (No.: CICC21746, Bacillus subtilis) preparation

[0046] First activate the Bacillus subtilis stored on the slant with sterilized solid ordinary medium...

Embodiment 2

[0072] Application of embodiment 2 soft-shelled turtle compound probiotic feed additive

[0073] The test site is in Yueyang Linxiang Breeding Base of Hunan Huangxin Aquatic Technology Co., Ltd., and the test object is Chinese soft-shelled turtle. The product of the present invention is used to feed Chinese soft-shelled turtle. The comparative analysis of the impact on its growth and benefits is as follows:

[0074]This experiment is based on commercial feed formula. A compound probiotic preparation was added to the basal feed, and the compound preparation was crushed to 60 meshes. Additives were set with 4 gradients (0mg / kg, 500mg / kg, 1000mg / kg, 2000mg / kg), and the test was divided into 4 groups, with 3 repetitions in each group, a total of 12 groups. The size of the pool in each group was 4m×10m×1.5m. 1200 animals were stocked in each pond. Feed 3 times a day (7:00-8:00, 11:00-12:00, 18:00-19:00), free to eat. The experiment lasted 20 weeks. Settle the weight gain rate, ...

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Abstract

The invention relates to a probiotic feed additive for Chinese softshell turtles and a preparation method of the probiotic feed additive. The probiotic feed additive is prepared from probiotics and carrier fillers, wherein the carrier fillers are sterilized wheat bran, and the probiotics include bacillus subtilis, bacillus natto, Lactobacillus casei and candida utilis with the ratio of (0.4-0.6):(0.4-0.6):(0.8-1.2):(0.8-1.2), and the probiotics are added in the aseptic carrier fillers to prepare probiotic powder. The probiotic feed additive can improve the growth performance of the Chinese softshell turtles, reduce the food coefficient, improve the immunological function and reduce the diseases, has the advantages of low cost, no pollution, no drug resistance, no residue and the like, and is environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of micro-ecological preparations and feed additives, and in particular relates to a Chinese soft-shelled turtle probiotic feed additive and a preparation method thereof. Background technique [0002] With the continuous expansion of market demand, the industrial culture of soft-shelled soft-shelled turtles has developed rapidly, but the high-density and intensive farming methods have changed their natural living habits, resulting in weakening of their disease resistance, and the increasing number of diseases has affected the Chinese soft-shelled turtle breeding industry. further development. In the modern breeding industry, adding antibiotics or chemically synthesized drugs to the feed plays an important role in preventing and curing diseases, promoting growth, saving feed, and improving the economic benefits of breeding. However, long-term use or excessive use will cause side effects such as immunosuppress...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16C12N11/02C12R1/125C12R1/07C12R1/245C12R1/72
Inventor 王晓清桂琳王子奥向秀成王璐明胡毅
Owner HUNAN AGRICULTURAL UNIV
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