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Direct fed microbial for prevention of shrimp disease

A technology of use and composition, applied in the field of direct-feeding microorganisms for preventing shrimp diseases, and can solve problems such as unsatisfactory development of antibiotics

Inactive Publication Date: 2018-09-28
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of antibiotics to control microbial development is not ideal due to the potential development of antibiotic resistance

Method used

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  • Direct fed microbial for prevention of shrimp disease
  • Direct fed microbial for prevention of shrimp disease
  • Direct fed microbial for prevention of shrimp disease

Examples

Experimental program
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Effect test

example 1

[0216] biological material deposit

[0217] Has been deposited under the terms of the Budapest Treaty at the Agricultural Research Service Patent Culture Collection (NRRL), Northern Regional Research Center, 1815 University Street, Peoria ( Peoria, Illinois, 61604, USA, has deposited the following biological material and is given the following accession numbers:

[0218] Preservation

Login ID

storage date

Bacillus subtilis O14VRQ

NRRL B-67221

January 8, 2016

Bacillus subtilis MF1048

NRRL B-67220

January 8, 2016

[0219] Bacillus subtilis strain O14VRQ was isolated on May 5, 2006 by Michael Frodyma from Novozymes North America, Inc. from soil samples in Virginia, USA.

[0220] Bacillus subtilis strain MF1048 was isolated on October 4, 2005 by Michael Frodyma from Novozymes North America, Inc. from a soil sample in Virginia, USA.

[0221] Said bacterial strains are deposited under the condition that during the pendency of thi...

example 2

[0223]Table 1 shows the relative survival of eight shrimp feed diets each containing one of the eight candidate strains of Bacillus. Shrimp fed Bacillus subtilis strain NRRL B-67221(014VRQ) or Bacillus subtilis strain NRRL B-67220(MF1048) had significantly greater survival than the other six Bacillus strains. Three tanks per treatment, ten shrimp per 10 gallon tank (30 shrimp per treatment) were used to compare shrimp survival over a 104 hour period. All shrimp were fed a standard amount of commercial shrimp feed pellets that had been treated with the indicated Bacillus organisms by surface spraying directly onto the pellets 24 hours prior to feeding. Specifically, a spore suspension in water of each treated bacterium is prepared, preferably at a concentration of 0.6x 10E8 CFU / ml to 1.2x 10E8 CFU / ml. The suspension was sprayed evenly onto the dry feed pellets (settling) at a preferred rate of 1.0 ml of spore suspension, to 10.0 g of feed pellets, and allowed to dry overnight ...

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Abstract

The present invention relates to Bacillus subtilis strains and their use for treatment of shrimp disease such as Early Mortality Syndrome.

Description

[0001] References to biological material depositories [0002] This application contains references to deposits of biological material, which are hereby incorporated by reference. See Example 1 for complete information. [0003] References to Sequence Listings [0004] This application contains a Sequence Listing in computer readable form. This computer readable form is hereby incorporated by reference. [0005] Index to sequence table: [0006] SEQ ID NO: 1 is the 16S rDNA of Bacillus subtilis strain O14VRQ with deposit accession number NRRL B-67221. [0007] SEQ ID NO: 2 is the 16S rDNA of Bacillus subtilis strain MF1048 with deposit accession number NRRL B-67220. technical field [0008] The present invention relates to Bacillus subtilis strains and their use for the treatment of shrimp diseases such as early death syndrome. Background technique [0009] Early Mortality Syndrome (EMS), also known as Acute Hepatopancreatic Necrosis (AHPND), is a relatively new shrimp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A23K10/18A23K50/80C12R1/125
CPCA61K35/742A23K10/18A23K50/80C12N1/205C12R2001/125
Inventor D·德拉霍斯S·丁佩里奥
Owner NOVOZYMES AS
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