Establishment process of judgment method for purebred similarity of dendrobium aphyllum, judgment standard and judgment method

Abstract

The invention relates to an establishment process of a judgment method for purebred similarity of dendrobium aphyllum, a judgment standard and the judgment method. The establishment process of the judgment method comprises the steps of S1, collecting dendrobium aphyllum samples consistent with a gene sequencing conclusion, and measuring the stem fourth internode length of each sample; S2, carryingout normality test on a stem fourth internode length variable of each sample; and S3, obtaining standard intervals: if a result obtained in the step S2 obeys normal distribution, obtaining a 95% confidence interval of a mean value and a 95% confidence interval of a standard deviation according to an overall calculation formula of the normal distribution, wherein the confidence intervals can serveas standard ranges for identifying the purity of unknown samples. The judgment standard established according to the actually collected data is that the 95% confidence interval of the mean value andthe 95% confidence interval of the standard deviation are (23.8377, 26.0996) and (4.4301, 6.0510) respectively, and the tested dendrobium aphyllum samples only need to be compared with the intervals.According to the method disclosed by the invention, the purity of the variety of the dendrobium aphyllum can be identified through the morphology-related characteristics of the dendrobium aphyllum, sothat the value of the artificially planted dendrobium aphyllum or whether the value of the artificially planted dendrobium aphyllum is existent or not can be simply judged; and the method is high inidentification accuracy, and has important practical significance.

Description

Technical field [0001] The present invention relates to the field of medicine and biology, and in particular to the establishment of a method for judging the closeness of pure species of Dendrobium latissimum, a judgment standard and a judgment method, that is, a method for establishing the correlation between the morphological characteristics of Dendrobium latissimum and the conclusion of gene sequencing and Methods of identifying the purity of the tested Dendrobium species. Background technique [0002] Dendrobium is a commonly used nourishing Chinese medicine, mainly from plants of the genus Dendrobium. Dendrobium is the largest genus of orchids, including Dendrobium laularii, Dendrobium fringe, Dendrobium officinale, Dendrobium nobile, Dendrobium densiflorum, Dendrobium guttata and many other varieties. Globally, there are about 1,100 kinds of Dendrobium, among which nearly 100 are found in my country. Dendrobium has a long history of medicinal use. It was listed as a top n...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of supplying Dendrobium.

However, the long-term artificial planting has also brought about false ones and substandard ones for Dendrobium nobile. This is because ① artificial planting has changed the growth environment of wild Dendrobium nobile; ② extensive application of various types of fertilizers, diseased , the application of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium nobile; ③Because there are many varieties of Dendrobium, the hybridization between the varieties makes the traits of its close relatives cross phenomenon; ④Others are uncontrollable Or unmeasurable factors, in summary, the medicinal ingredients of some artificially planted Dendrobium nobile have changed, and even important medicinal ingredients have disappeared. The gene sequence is also substantially different from the original wild gene sequence

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