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A method for analyzing the role of Rab8 in regulating Klotho expression in non-small cell lung cancer

A technology for non-small cell lung cancer and analysis method, which is applied in the field of analysis of the role of Rab8 in regulating Klotho expression in non-small cell lung cancer, and achieves the effect of improving the distribution level on the membrane surface and inhibiting migration.

Active Publication Date: 2021-06-22
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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Problems solved by technology

[0003] At present, there are still many unknowns about the function of Rab8 and the signaling pathway related to Rab8

Method used

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  • A method for analyzing the role of Rab8 in regulating Klotho expression in non-small cell lung cancer
  • A method for analyzing the role of Rab8 in regulating Klotho expression in non-small cell lung cancer
  • A method for analyzing the role of Rab8 in regulating Klotho expression in non-small cell lung cancer

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Embodiment Construction

[0041] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0042] Previous studies have confirmed that Rab8 and Klotho interact with each other in non-small cell lung cancer and co-localize in subcellular cells, and Rab8 can promote the expression of Klotho protein membrane surface. Next, we want to further explore the specific mechanism of Rab8 regulation of Klotho transport metabolism. mechanism, and the resulting changes in the biological behavior of non-small cell lung cancer.

[0043] A method for analyzing the m...

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Abstract

The invention discloses a method for analyzing the effect of Rab8 on regulating the expression of Klotho in non-small cell lung cancer, including analyzing the effect of Rab8 on regulating the expression of Klotho on the membrane surface through the post-translation link, analyzing the Wnt signaling pathway and the regulation of the expression of Klotho on the membrane surface by Rab8. The role in EMT and analysis of the role of Rab8 in regulating Klotho membrane surface expression in non-small cell lung cancer cell function. The present invention finds for the first time that Rab8 binds to Klotho, promotes its transport to the membrane surface through the pathway after protein biosynthesis, improves the distribution level of Klotho membrane surface, inhibits Wnt / β-catenin signal activation, and inhibits epithelial-mesenchymal transition of cells, thereby inhibiting Lung cancer cell migration and invasion. Therefore, Rab8 has the function of actively regulating Klotho.

Description

technical field [0001] The invention relates to an analysis method of protein interaction and related action mechanism, specifically a method for analysis of the role of Rab8 in regulating Klotho expression in non-small cell lung cancer, belonging to the field of biological applications. Background technique [0002] In recent years, researchers are increasingly interested in the study of Rab8. Rab8 was originally discovered from melanoma cells and considered to be a transforming gene. It is currently believed that Rab8 is involved in the transport of newly synthesized proteins in polarized cells, thereby participating in the regulation of cell shape and in response to different cellular signals. Rab8 plays a tandem role in membrane trafficking and cytoskeleton dynamics by reorganizing actin and microtubules, thereby having important effects on cell morphology. It has been reported that Rab8 is involved in the formation and material transport of primary cilia. It was rece...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C12Q1/6851
CPCC12Q1/6851G01N33/68C12Q2531/113
Inventor 吴剑卿陈波赵卫红
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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