Cultivation method for increasing content of triterpenoids in chaga
A technology of triterpenoids in mushrooms and a culturing method is applied in the cultivation field of increasing the content of triterpenoids in Chaga mushrooms, and can solve the problems of slow growth rate, low product yield, low component ratio and the like
Active Publication Date: 2020-11-20
TIMING PHARMA
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Problems solved by technology
However, at the present stage, the solid-state culture technology of Chaga has lower yield and slower growth rate, and the ratio of active ingredients triterpenoids contained is lower than that of naturally occurring wild Chaga
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[0040]As shown in step S100 of Fig. 1, in this embodiment, the chaga strain is first inoculated on the solid medium, wherein the solid medium is placed in a solid culture bottle, and the bottom area of the solid culture bottle is 220 square centimeters . Furthermore, the aforementioned solid medium is preferably mixed with mung bean powder, glucose, birch wood chips, agar and water in the following ratio: 11.8% mung bean powder, 9.7% birch wood chips, 1.3% glucose, 9.5% agar And 67.7% of water, and prepared by high temperature sterilization. Next, the Chaga strain is cultured in a light environment at normal temperature to produce Chaga mycelium, wherein the normal temperature refers to controlling the temperature of the culture environment between 15°C and 25°C, and preferably 25°C.
[0041] Subsequently, if figure 1 As shown in step S102, when the chaga mycelium is cultivated to an area between 40 cm2 and 60 cm2, the first nutrient solution is placed on the solid medium ...
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The invention discloses a solid culture method for increasing Triterpenoids content content of inonotus obliquus. The solid culture method includes steps of inoculation, first nutrient solution adding, first culture environment adjustment, second nutrient solution adding, second culture environment adjustment and harvesting. The solid culture method specifically includes: firstly, performing inoculation of an inonotus obliquus strain in a solid culture medium to culture inonotus obliquus mycelia; secondly, when the inonotus obliquus mycelia reach a first preset area, adding a first nutrient solution; thirdly, when the inonotus obliquus mycelia reach a second preset area, reducing brightness and temperature of a culture environment; then after adding of a second nutrient solution, recovering brightness and temperature of the culture environment, and harvesting when the inonotus obliquus mycelia reach a third preset area. Therefore, the content of Triterpenoids in products of inonotus obliquus mycelia can be effectively increased.
Description
technical field [0001] The invention relates to a cultivation method of chaga, in particular to a cultivation method for increasing the content of triterpenoids in chaga. Background technique [0002] Chaga (Inonotus obliquus) is a folk medicinal fungus mainly distributed in Russia and Eastern Europe. Its Chinese aliases include Chaga, Chaga, Inonotus obliquus, Siberian Ganoderma, Chaga and tree mushrooms. The scientific classification belongs to the kingdom Fungi, Basidiomycota, Agaricomycetes, Hymenochaetales, Hymenochaetaceae, and Inonotus. [0003] In recent years, studies have shown that the polysaccharides and triterpenoids contained in chaga can effectively activate the immune system and inhibit the proliferation of viruses, and have the effect of preventing breast cancer, liver cancer, uterine cancer, gastric cancer, diabetes and high blood pressure , its nutritional and medicinal value has gradually attracted people's attention, especially the triterpenoids in it h...
Claims
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IPC IPC(8): A01G18/20A01G18/68A01G18/69
Inventor 王伯纶
Owner TIMING PHARMA